Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 17, Number 5—May 2011
Letter

Rare Rotavirus Strains in Children with Severe Diarrhea, Malaysia

Ling-Sing Ch’ng, Way S. Lee, and Carl D. KirkwoodComments to Author 
Author affiliations: Author affiliations: Murdoch Childrens Research Institute, Parkville, Victoria, Australia (L.-S. Ch’ng, C.D. Kirkwood); University of Melbourne, Melbourne, Victoria, Australia (L.-S. Ch’ng, C.D. Kirkwood); University of Malaya, Kuala Lumpur, Malaysia (W.S. Lee)

Main Article

Figure

Phylogenetic relationship of nucleotide sequences of genes encoding the outer capsid proteins VP7 and VP4 from G3P[9] rotavirus strains. A) Evolutionary relationship of G3 VP7 nucleotide sequences. B) Evolutionary relationship of P[9] VP4 nucleotide sequences. The evolutionary relationship was inferred by using the neighbor-joining method. The percentages of the bootstrap test (2,000 replicates) are shown next to the branches. The evolutionary distances were computed by using the maximum-composi

Figure. Phylogenetic relationship of nucleotide sequences of genes encoding the outer capsid proteins VP7 and VP4 from G3P[9] rotavirus strains. A) Evolutionary relationship of G3 VP7 nucleotide sequences. B) Evolutionary relationship of P[9] VP4 nucleotide sequences. The evolutionary relationship was inferred by using the neighbor-joining method. The percentages of the bootstrap test (2,000 replicates) are shown next to the branches. The evolutionary distances were computed by using the maximum-composite likelihood method and represent the number of base substitutions per site. Phylogenetic analysis was conducted by using MEGA version 4 (10). The labeling of the taxon corresponds to host name, followed by G-type and/or P-type, strain name, place of origin, and year of isolation. The letter S or P after the year of isolation indicates submission or published year of the sequence in the National Center for Biotechnology Information database. Arrows indicate G3P[9] isolate identified in this study. Scale bars indicate nucleotide substitutions per site.

Main Article

References
  1. Parashar  UD, Burton  A, Lanata  C, Boschi-Pinto  C, Shibuya  K, Steele  D, Global mortality associated with rotavirus disease among children in 2004. J Infect Dis. 2009;200:S915. DOIPubMedGoogle Scholar
  2. De Grazia  S, Giammanco  GM, Potgieter  AC, Matthijnssens  J, Bányai  KN, Platia  MA, . Unusual assortment of segments in 2 rare human rotavirus genomes. Emerg Infect Dis. 2010;16:85962.PubMedGoogle Scholar
  3. Griffin  DD, Nakagomi  T, Hoshino  Y, Nakagomi  O, Kirkwood  CD, Parashar  UD, Characterization of nontypeable rotavirus strains from the United States: identification of a new rotavirus reassortant (P2A[6],G12) and rare P3[9] strains related to bovine rotaviruses. Virology. 2002;294:25669. DOIPubMedGoogle Scholar
  4. Inoue  Y, Kitahori  Y. Rare group a rotavirus P[9]G3 isolated in Nara Prefecture, Japan. Jpn J Infect Dis. 2006;59:13940.PubMedGoogle Scholar
  5. Khamrin  P, Maneekarn  N, Peerakome  S, Tonusin  S, Phan  TG, Okitsu  S, Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis. J Med Virol. 2007;79:84351. DOIPubMedGoogle Scholar
  6. Wang  Y-H, Kobayashi  N, Zhou  X, Nagashima  S, Zhu  Z-R, Peng  J-S, Phylogenetic analysis of rotaviruses with predominant G3 and emerging G9 genotypes from adults and children in Wuhan, China. J Med Virol. 2009;81:3829. DOIPubMedGoogle Scholar
  7. Rasool  NB, Larralde  G, Gorziglia  MI. Arch Virol. 1993;133:27582.Determination of human rotavirus VP4 using serotype-specific cDNA probes. DOIPubMedGoogle Scholar
  8. Iturriza-Gómara MI, Cubitt D, Desselberger U, Gray J. Amino acid substitution within the VP7 protein of G2 rotavirus strains associated with failure to serotype. J Clin Microbiol. 2001;39:3796–8.DOIGoogle Scholar
  9. Simmonds  MK, Armah  G, Asmah  R, Banerjee  I, Damanka  S, Esona  M, New nucleotide primers for p-typing of rotavirus strains: strategies for typing previously untypeable strains. J Clin Virol. 2008;42:36873. DOIPubMedGoogle Scholar
  10. Tamura  K, Dudley  J, Nei  M, Kumar  S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24:15969. DOIPubMedGoogle Scholar

Main Article

Page created: August 14, 2011
Page updated: August 14, 2011
Page reviewed: August 14, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external