Rare Rotavirus Strains in Children with Severe Diarrhea, Malaysia
Ling-Sing Ch’ng, Way S. Lee, and Carl D. Kirkwood
Author affiliations: Author affiliations: Murdoch Childrens Research Institute, Parkville, Victoria, Australia (L.-S. Ch’ng, C.D. Kirkwood); University of Melbourne, Melbourne, Victoria, Australia (L.-S. Ch’ng, C.D. Kirkwood); University of Malaya, Kuala Lumpur, Malaysia (W.S. Lee)
Figure. Phylogenetic relationship of nucleotide sequences of genes encoding the outer capsid proteins VP7 and VP4 from G3P rotavirus strains. A) Evolutionary relationship of G3 VP7 nucleotide sequences. B) Evolutionary relationship of P VP4 nucleotide sequences. The evolutionary relationship was inferred by using the neighbor-joining method. The percentages of the bootstrap test (2,000 replicates) are shown next to the branches. The evolutionary distances were computed by using the maximum-composite likelihood method and represent the number of base substitutions per site. Phylogenetic analysis was conducted by using MEGA version 4 (10). The labeling of the taxon corresponds to host name, followed by G-type and/or P-type, strain name, place of origin, and year of isolation. The letter S or P after the year of isolation indicates submission or published year of the sequence in the National Center for Biotechnology Information database. Arrows indicate G3P isolate identified in this study. Scale bars indicate nucleotide substitutions per site.
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