José Gomes Temporão, Gerson Oliveira Penna, Eduardo Hage Carmo, Giovanini Evelim Coelho, Raimunda do Socorro Silva Azevedo, Márcio Roberto Teixeira Nunes, and Pedro F.C. Vasconcelos
Author affiliations: Author affiliations: Ministry of Health Brasília, Brasilia, Brazil (J.G. Temporão, G.O. Penna); University of Brasília, Brasilia (G.O. Penna); Secretariat of Health Surveillance, Brasilia (E.H. Carmo, G.E. Coelho); Evandro Chagas Institute, Ananindeua, Brazil (R. do Socorro Silva Azevedo, M.R.T. Nunes, P.F. da Costa Vasconcelos)
Figure. A) Boa Vista, Roraima State, Brazil, showing the districts where dengue virus type 4 (DENV-4) was isolated. 1, Liberdade; 2, Buritis; 3, Cauamé; 4, Santa Tereza; 5, Cidade Satélite; 6, Dr. Silvio Leite; 7, Joquel Clube; 8, Sen. Hélio Campos. B) Phylogenetic analysis of the DENV-4 envelope gene sequences (in boldface) constructed by using the neighbor-joining method, showing the cluster formed by Boa Vista and Venenzuela DENV-4 genotype I strains. Bootstrap values were set for 1,000 replicates and are placed over each main node of the tree. ROR1982 represents the DENV-4 isolated in Roraima State during the 1982 epidemic. A to B1–B4 represent genotype I subclades. Thailand 1978 and Thailand 1985 strains represent the Asian genotype II. Scale bar corresponds to 1% nucleotide sequence divergence.
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