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Volume 17, Number 5—May 2011

Dispatch

Novel Bluetongue Virus Serotype from Kuwait

Sushila Maan, Narender S. Maan, Kyriaki Nomikou, Carrie Batten, Frank Antony, Manjunatha N. Belaganahalli, Attia Mohamed Samy, Ammar Abdel Reda, Sana Ahmed Al-Rashid, Maha El Batel, Chris A.L. Oura, and Peter P.C. MertensComments to Author 
Author affiliations: Author affiliations: Institute for Animal Health, Woking, UK (S. Maan, N.S. Maan, K. Nomikou, C. Batten, F. Antony, M.N. Belaganahalli, C.A.L. Oura, P.P.C. Mertens); Public Authority of Agriculture Affairs and Fish Resources, Kuwait City, Kuwait (A.M. Samy, A.A. Reda, S.A. Al-Rashid, M. El Batel); Cairo University, Cairo, Egypt (A.M. Samy)

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Figure 1

Electrophoretic analysis of genomic double-stranded RNAs from the Orbivirus species and mammalian orthoreoviruses. Bluetongue virus double stranded RNA preparations were analyzed by electrophoresis in a 1% agarose gel containing 0.5 μg/mL ethidium bromide and visualized by exposure to ultraviolet light. Genome segments are numbered, in order of decreasing molecular weight. DNA markers were run (lanes M) to enable estimation of molecular weights. Lane 1, orthoreovirus (MOR2004/01); 2, equine ence

Figure 1. Electrophoretic analysis of genomic double-stranded RNAs from the Orbivirus species and mammalian orthoreoviruses. Bluetongue virus double stranded RNA preparations were analyzed by electrophoresis in a 1% agarose gel containing 0.5 μg/mL ethidium bromide and visualized by exposure to ultraviolet light. Genome segments are numbered, in order of decreasing molecular weight. DNA markers were run (lanes M) to enable estimation of molecular weights. Lane 1, orthoreovirus (MOR2004/01); 2, equine encephalosis virus (EEV-1/RSA1976/03); 3, African horse sickness virus (AHSV-1/RSArrah/01); 4, Palyam virus (PALV-SUD1982/03); 5, epizootic hemorrhagic disease virus (EHDV-4/ NIG1968/01); 6, bluetongue virus (BTV-15/RSArrrr/15); 7, bluetongue virus (BTV-26/KUW2010/02); 8, Tilligerry virus (TILV-AUS1978/03); 9, Chobar Gorge virus (CGV).

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