Chikungunya Virus, Southeastern France
Figure. Phylogenetic relationships among chikungunya virus isolates from cases of chikungunya fever in France, based on complete E2-6K-E1 nucleotide sequence (2,771 nt) analysis. Gray shading indicates imported and autochthonous strains. Sequence alignments were performed by using BioNumerics version 5.1 (www.applied-maths.com). Phylogenetic analysis was inferred by using the maximum-likelihood method as implemented in MEGA version 5 software (www.megasoftware.net). Bootstrap support values (1,000 replicates) are indicated at major nodes. The sequence of the strains from France described in this study has been deposited in GenBank (accession number pending); other sequences were retrieved from GenBank. Scale bar indicates number of base substitutions per site. ECSA, east/central/south Asia.