Possible Novel Nebovirus Genotype in Cattle, France
Figure 1. Nebovirus phylogenetic tree based on the deduced 167-aa–length sequences covering the 3′ end polymerase region. Possible novel strain is shown in boldface. Sequence alignments and clustering were performed by the unweighted-pair group method by using arithmetic average with Bionumerics software (Applied Maths, Sint-Martens-Latem, Belgium). Bootstrap values calculated with 1,000 replicate trees are given at each node when >70%. Collapsed branches are made up of the following nebovirus strains from France: *A042/06/FR, A051/05/FR, A058/05/FR, A130–2/06/FR, A143–2/06/FR, A311–2/08/FR, A381/08/FR, A445–2/08/FR, and A448/08/FR; †A281/07/FR, A355/07/FR, A356/07/FR, A364/08/FR, and A365/08/FR. Partial polymerase sequences of the French nebovirus strains were submitted to the GenBank database under accession nos. GU259537–GU259569 (A complete list is available from the authors.) GenBank accession nos. of calicivirus reference strains used in this tree are the following: AY082890 (CV23-OH/00/US), AY082891 (Nebraska/80/US), NC_007916 (Newbury1/76/UK), AF097917 (Newbury2/76/UK), DQ228162 (PenrithC39/00/UK), DQ228160 (Penrith142/00/UK), DQ228161 (Penrith143/00/UK), DQ228157 (Penrith150/00/UK), DQ228165 (Starcross93/00/UK), and DQ228164 (Starcross117/00/UK). Underlined strain is an outgroup. Scale bar indicates the percentage of similarities between strains.