Possible Novel Nebovirus Genotype in Cattle, France
Figure 2. Nebovirus phylogenetic tree based on the deduced 549-aa–length sequences of the complete capsid. Possible novel strain is shown in boldface. Sequence alignments and clustering were performed by the unweighted-pair group method by using arithmetic average with Bionumerics software (Applied Maths, Sint-Martens-Latem, Belgium). Bootstrap values calculated with 1000 replicate trees are given at each node when >70%. Capsid sequences of the French nebovirus strains were submitted to the GenBank database under the following accession nos.: GU259542 (DijonA058/05/FR), FJ687386 (DijonA216/06/FR), GU259551 (DijonA290–2/07/FR), GU259553 (DijonA311–2/08/FR), GU259556 (DijonA364/08/FR), GU259561 (DijonA386/08/FR), and GU259569 (DijonA448/08/FR). GenBank accession nos. of Calicivirus reference strains used in this tree are: AY082890 (CV23-OH/00/US), AY549169 (CV519-OH/02/US), AY549170 (CV526-OH/02/US), AY549171 (CV531-OH/02/US), EF528561 (MA274/04/KOR), EF528564 (MA362/04/KOR), EF528569 (MA729/05/KOR), AY082891 (Nebraska/80/US), NC_007916 (Newbury1/76/UK), and AF097917 (Newbury2/76/UK). Underlined strain is an outgroup. Scale bar indicates the percentage of similarities between strains.