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Volume 17, Number 6—June 2011

Letter

Easy Diagnosis of Invasive Pneumococcal Disease

Laura Selva, Xavier Krauel, Roman Pallares, and Carmen Muñoz-AlmagroComments to Author 
Author affiliations: Author affiliations: University Hospital Sant Joan de Déu, Barcelona, Spain (L. Selva, X. Krauel, C. Muñoz-Almagro); Saint John of God Hospital, Lunsar, Sierra Leone (X. Krauel); Bellvitge Hospital, Barcelona (R. Pallares); University of Barcelona, Barcelona (R. Pallares)

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Table

Sensitivity of real-time PCR for detecting Streptococcus pneumoniae ply or wzg genes or a specific gene for molecular serotype from fresh or dried blood spot samples*

Gene or serotype Serial dilutions correctly detected, CFU equivalent/PCR†
Fresh blood Dried blood spot
ply gene 1.101–1.103 1.101–1.103
wzg gene 1.101–1.103 1.101–1.103
Serotype 1 1.101–1.103 1.101–1.103
Serotype 5 1.101–1.103 1.101–1.103
Serotype 19A 1.101–1.103 1.101–1.103
Serotype 19F 1.101–1.103 1.101–1.103
Serotype 14 1.101–1.103 1.101–1.103
Serotype 3 1.102–1.103 1.101–1.103
Serotype 7F 1.101–1.103 1.101–1.103
Serotype 4 1.101–1.103 1.101–1.103
Serotype 6A 1.101–1.103 1.102–1.103
Serotype 6B 1.101–1.103 1.101–1.103
Serotype 8 1.101–1.103 1.102–1.103
Serotype 9N 1.101–1.103 1.101–1.103
Serotype 18C 1.101–1.103 1.101–1.103
Serotype 23A 1.101–1.103 1.101–1.103
Serotype 23F 1.101–1.103 1.101–1.103

*Serial dilutions (10–1,000 CFU equivalent/PCR) of 15 pneumococcus cultures mixed with 15 negative-control blood samples were analyzed. Boldface indicates results that differ from others.
†10 CFU equivalent/PCR = 1,000 CFU equivalents/mL blood.

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