Volume 17, Number 6—June 2011
Letter
Easy Diagnosis of Invasive Pneumococcal Disease
Laura Selva, Xavier Krauel, Roman Pallares, and Carmen Muñoz-Almagro
Table
Sensitivity of real-time PCR for detecting Streptococcus pneumoniae ply or wzg genes or a specific gene for molecular serotype from fresh or dried blood spot samples*
| Gene or serotype | Serial dilutions correctly detected, CFU equivalent/PCR† |
|
|---|---|---|
| Fresh blood | Dried blood spot | |
| ply gene | 1.101–1.103 | 1.101–1.103 |
| wzg gene | 1.101–1.103 | 1.101–1.103 |
| Serotype 1 | 1.101–1.103 | 1.101–1.103 |
| Serotype 5 | 1.101–1.103 | 1.101–1.103 |
| Serotype 19A | 1.101–1.103 | 1.101–1.103 |
| Serotype 19F | 1.101–1.103 | 1.101–1.103 |
| Serotype 14 | 1.101–1.103 | 1.101–1.103 |
| Serotype 3 | 1.102–1.103 | 1.101–1.103 |
| Serotype 7F | 1.101–1.103 | 1.101–1.103 |
| Serotype 4 | 1.101–1.103 | 1.101–1.103 |
| Serotype 6A | 1.101–1.103 | 1.102–1.103 |
| Serotype 6B | 1.101–1.103 | 1.101–1.103 |
| Serotype 8 | 1.101–1.103 | 1.102–1.103 |
| Serotype 9N | 1.101–1.103 | 1.101–1.103 |
| Serotype 18C | 1.101–1.103 | 1.101–1.103 |
| Serotype 23A | 1.101–1.103 | 1.101–1.103 |
| Serotype 23F | 1.101–1.103 | 1.101–1.103 |
*Serial dilutions (10–1,000 CFU equivalent/PCR) of 15 pneumococcus cultures mixed with 15 negative-control blood samples were analyzed. Boldface indicates results that differ from others.
†10 CFU equivalent/PCR = 1,000 CFU equivalents/mL blood.
