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Volume 17, Number 6—June 2011

Dispatch

Novel Reassortant Highly Pathogenic Avian Influenza (H5N5) Viruses in Domestic Ducks, China

Min Gu, Wenbo Liu, Yongzhong Cao, Daxin Peng, Xiaobo Wang, Hongquan Wan, Guo Zhao, Quangang Xu, Wei Zhang, Qingqing Song, Yanfang Li, and Xiufan LiuComments to Author 
Author affiliations: Author affiliation: Yangzhou University College of Veterinary Medicine, Yangzhou, People’s Republic of China

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Table 1

Characteristics of 2 novel avian influenza (H5N5) viruses isolated from domestic ducks, China, December 2008–January 2009*

Virus Characteristics
Virus replication in experimentally infected mice,
no. virus-positive mice/no. tested mice (mean titer + SD)†
IVPI EID50 TCID50 MLD50 Tissue dpi 2 dpi 4 dpi 6 dpi 8 dpi 10
008
2.6
108.5
108.6
104.0

Lung 1/2 (3.2 ± 0) 2/2 (3.8 ± 0.4) 2/2 (4.7 ± 0.4) 2/2 (4.4 ± 0.5) 0/2
Brain 0/2 0/2 0/2 2/2 (3.8 ± 1.2) 1/2 (3.4 ± 0)
Heart 1/2 (2.0 ± 0) 2/2 (2.6 ± 0.5) 2/2 (3.1 ± 0.1) 0/2 0/2
Spleen 0/2 0/2 0/2 0/2 1/2 (2.3 ± 0)
Liver 0/2 0/2 1/2 (2.2 ± 0) 0/2 0/2
Kidney
0/2
0/2
0/2
0/2
0/2
031 2.5 108.3 108.5 105.4 Lung 1/2 (3.0 ± 0) 1/2 (3.3 ± 0) 2/2 (4.1 ± 0.2) 2/2 (4.5 ± 0.3) 1/2 (2.6 ± 0)
Brain 0/2 0/2 0/2 1/2 (3.4 ± 0) 0/2
Heart 0/2 1/2 (2.5 ± 0) 1/2 (3.0 ± 0) 0/2 0/2
Spleen 0/2 0/2 0/2 0/2 0/2
Liver 0/2 0/2 0/2 0/2 0/2
Kidney 0/2 0/2 1/2 (2.1 ± 0) 0/2 0/2

*EID50, 50% egg infectious dose; IVPI, intravenous pathogenicity index (determined in chickens); TCID50, 50% tissue culture infectious dose (determined in MDCK cells); MLD50, 50% lethal dose in mice (expressed as the EID50 value corresponding to 1 LD50); dpi, day postinoculation; 008, A/duck/eastern China/008/2008; 031, A/duck/eastern China/031/2009.
†Mice were inoculated with 103.5 EID50; 2 infected mice were euthanized every other day for virus detection. Virus titers were expressed as log10 EID50/g.
‡Influenza A viruses with MLD50 <103.0 EID50 were considered of high pathogenicity, MLD50 >106.5 EID50 were considered of low pathogenicity (4).

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