Emerging Infectious Disease ISSN: 1080-6059

Macrolide Resistance in Mycoplasma pneumoniae, Israel, 2010

Diana Averbuch, Carlos Hidalgo-Grass, Allon E. Moses, Dan Engelhard, and Ran Nir-Paz

Author affiliations: Author affiliation: Hadassah–Hebrew University Medical Center, Jerusalem, Israel

Figure 2

Real-time PCR high-resolution melting assay. A) High-resolution melt profiles are shown for wild-type (WT) Mycoplasma pneumoniae A (A2063A), macrolide resistance mutation G (A2063G) sample, and the mixed genotype sample in the normalized graph mode. B) Temperature-shifted difference graph demonstrates the deviations between WT, resistant, and mixed samples. The WT isolate has been selected to normalize the temperature shift graph and displays the deviation of samples from it.

Figure 2. Real-time PCR high-resolution melting assay. A) High-resolution melt profiles are shown for wild-type (WT) Mycoplasma pneumoniae A (A2063A), macrolide resistance mutation G (A2063G) sample, and the mixed genotype sample in the normalized graph mode. B) Temperature-shifted difference graph demonstrates the deviations between WT, resistant, and mixed samples. The WT isolate has been selected to normalize the temperature shift graph and displays the deviation of samples from it.

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¹These authors contributed equally to this article.

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