Figure 2. Analyses of Salmonella enterica serotype Choleraesuis isolates from Chang Gung Memorial Hospital, 1999–2010. A) Pulsed-field gel electrophoresis patterns. Lanes 1 and 10, DNA size markers demonstrated by a λ DNA concatemer standard and S. enterica ser. Braenderup H9812, respectively; lanes 2 to 9, S. enterica ser. Choleraesuis SC-B67, SC-B104, SC-B93, SC-B98, SC-B131, SC-B132, SC-B134, and SC-B136. B) Plasmid analysis and C) DNA–DNA hybridization. Probes for DNA–DNA hybridization of lanes 1–6, 7–12, and 13–16 were prepared from amplicons of spvC, Rep_3 replicon of pSC138 in SC-B67, and repI1, respectively; lanes 1 and 7, S. enterica ser. Choleraesuis OU7529 containing 2 plasmids of known sizes, 50 kb and 90 kb, was used as the size marker; lanes 2 and 8, SC-B67; lanes 3, 9, and 13, SC-B134; lanes 4, 10, and 14, SC-B136; lanes 5, 11, and 15, Escherichia coli J53/pSC-B134; lanes 6, 12, and 16, E. coli J53/pSC-B136.