Figure 2. Analyses of Salmonellaenterica serotype Choleraesuis isolates from Chang Gung Memorial Hospital, 1999–2010. A) Pulsed-field gel electrophoresis patterns. Lanes 1 and 10, DNA size markers demonstrated by a λ DNA concatemer standard and S. enterica ser. Braenderup H9812, respectively; lanes 2 to 9, S. enterica ser. Choleraesuis SC-B67, SC-B104, SC-B93, SC-B98, SC-B131, SC-B132, SC-B134, and SC-B136. B) Plasmid analysis and C) DNA–DNA hybridization. Probes for DNA–DNA hybridization of lanes 1–6, 7–12, and 13–16 were prepared from amplicons of spvC, Rep_3 replicon of pSC138 in SC-B67, and repI1, respectively; lanes 1 and 7, S. enterica ser. Choleraesuis OU7529 containing 2 plasmids of known sizes, 50 kb and 90 kb, was used as the size marker; lanes 2 and 8, SC-B67; lanes 3, 9, and 13, SC-B134; lanes 4, 10, and 14, SC-B136; lanes 5, 11, and 15, Escherichia coli J53/pSC-B134; lanes 6, 12, and 16, E. coli J53/pSC-B136.
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.