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Volume 17, Number 6—June 2011

Dispatch

Increasing Ceftriaxone Resistance in Salmonellae, Taiwan

Lin-Hui Su, Wen-Shin Teng, Chyi-Liang Chen, Hao-Yuan Lee, Hsin-Chieh Li, Tsu-Lan Wu, and Cheng-Hsun ChiuComments to Author 
Author affiliations: Author affiliations: Chang Gung Memorial Hospital, Taoyuan, Taiwan (L.-H. Su, H.-C. Li, T.-L. Wu); Chang Gung University College of Medicine, Taoyuan (L.-H. Su, W.-S. Teng, H.-Y. Lee, T.-L. Wu, C.-H. Chiu); Chang Gung Children’s Hospital, Taoyuan (W.-S. Teng, C.-L. Chen, H.-Y. Lee, C.-H. Chiu)

Main Article

Table 1

Characteristics of the resistant Salmonella enterica isolates from 17 patients at Chang Gung Memorial Hospital, Taiwan*

Serotype and isolate (no. patients) Year Type of specimen Susceptibility profile† Puls‡ Plasmid profile, kb§ DNA–DNA hybridization¶
PCR sequencing#
spvC rep FIIA/FIB Rep_3 gyrA
parC
Ser(83) D(87) Ser(80)
Choleraesuis
SC-B67 2002 Blood R/R/R C-1-a 50, 138 50 50 138 F N I
SC-B104 (1) 2003 Blood R/R/R C-1-b 50, 150 50 50 150 F N I
SC-B93 (2) 2003 Blood R/R/R C-1-b 50, 115 50 50 50,115 F N I
SC-B98 (3) 2004 Blood R/R/R C-1-c 50, 138 50 50 138 F N I
NA (4) 2004 Pus R/R/R NA NA NA NA NA NA NA NA
SC-B131 (5) 2004 Blood R/R/R C-1-d 50, 138 50 50 138 F N I
SC-B132 (6) 2004 Blood R/R/R C-1-b 50, 150 50 50 150 F N I
NA (7) 2005 Pus R/S/R NA NA NA NA NA NA NA NA
SC-B134 (8) 2007 Blood R/S/R† C-1-e 40, 65, 105 65 65 40 Ser N Ser
SC-B136 (9) 2008 Blood R/R/R C-2 115, 138 115 115 115 F N I
NA (10)
2009
Pus
R/S/R
NA
NA
NA
NA
NA

NA
NA

NA
Typhimurium var. Copenhagen SB-5
2010
Feces
R/R/R
B-1-a
7, 125, 180, 260
Neg
Neg
Neg

Ser
D

Ser
Typhimurium
SB-28 2010 Urine R/R/S B-2 115, 210 Neg Neg Neg Ser D Ser
SB-151 2010 Feces S/S/S B-1-b 85 Neg Neg Neg Ser D Ser
SB-193
2010
Feces
R/R/S
B-2
105, 210
Neg
Neg
Neg

Ser
D

Ser
Agona SB-105
2010
Feces
R/S/S
B-3
95
Neg
Neg
Neg

Ser
D

Ser
Enteritidis SD-166 2010 Feces R/S/S D-1 45, 60, 95 60 Neg Neg Ser D Ser

*Puls, pulsotype; R, resistant; F, phenylalanine; N, asparagine; I, isoleucine; NA, not available; S, susceptible; ser, serine; neg, negative reaction; var., variant; D, aspartic acid.
†Antimicrobial drug susceptibility to chloramphenicol, trimethoprim/sulfamethoxazole, and quinolones. Results were the same for the 2 quinolones (nalidixic acid and ciprofloxacin) tested, except that SC-B134 was resistant to nalidixic acid and susceptible to ciprofloxacin. All isolates were resistant to ampicillin and ceftriaxone.
‡Pulsed-field gel electrophoresis; pulsotypes are expressed as serogroup-major type-subtype.
§Plasmids harboring the blaCMY-2-carrying Tn6092 element are shown in boldface. IncI1 plasmids are underlined. Both are evidenced by DNA–DNA hybridization.
¶The size (kb) of the plasmid showing positive results in the respective DNA–DNA hybridization experiments is indicated. Rep_3, replicon of pSC138, the Tn6092-containing resistant plasmid of strain SC-B67.
#Amino acid changes compared with the quinolone resistance–determining regions of gyrA (codons 83 and 87) and parC (codon 80) in S. enterica
serotype Typhimurium LT2. No mutation was found in gyrB and parE.

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