Volume 17, Number 7—July 2011
Dispatch
Clonal Genotype of Geomyces destructans among Bats with White Nose Syndrome, New York, USA
Sunanda S. Rajkumar, Xiaojiang Li, Robert J. Rudd, Joseph C. Okoniewski, Jianping Xu, Sudha Chaturvedi, and Vishnu Chaturvedi
Table 2
Geomyces destructans and G. pannorum target gene fragments used for multiple gene genealogic analyses, New York, USA
| Gene* | Homology (GenBank accession no.) | Amplicon size/ sequence used for comparison, bp | Primer sequence, 5′ → 3′† | G. destructans/G. pannorum GenBank accession nos. |
|---|---|---|---|---|
| ALR |
Penicillium marneffei (XP_002152078.1) |
654/534 |
V1905 (f): CGGAGTGAGATTTATGACGGC | HQ834314–HQ834329/HQ834330 |
| V1904 (r): CGTCCATCCCAGACGTTCATC |
||||
| Bpntase |
Glomerella graminicola (EFQ33509.1) |
921/745 |
V1869 (f): TCAGACGGACTCGGAGGGCAAG | HQ834331–HQ834346/HQ834347 |
| V1926 (r): TCGGTTACAGAGCCTCAGTCG |
||||
| DHC1 |
Sordaria macrospora (CBI53717.1) |
597/418 |
V1906 (f): GGATGATTCGGTCACCAAACAG | HQ834348–HQ834363/HQ834364 |
| V1907 (r): ACAGCAAACACAGCGCTGCAAG |
||||
| GPHN |
Ajellomyces capsulatus (EEH06836.1) |
659/525 |
V1918 (f): CACTATTACATCGCCAGGCTC | HQ834365–HQ834380/HQ834381 |
| V1919 (r): CTAAACGCAGGCACTGCCTC |
||||
| PCS |
A. capsulatus
(EEH08767.1) |
920/749 |
V1929 (f): AGGCTGCGATTGCTGAGTGC | HQ834382–HQ834397/HQ834398 |
| V1873 (r): CCTTATCCAGCTTTCCTTGGTC |
||||
| POB3 |
Pyrenophora tritici-repentis (XP_001937502.1) |
653/417 |
V1908 (f): CACAGTGGAGCAAGGCATCC | HQ834399–HQ834414/HQ834415 |
| V1909 (r): ACATACCTAGGCGTCAAGTGC |
||||
| SRP72 |
A. dermatitidis (EEQ90678.1) |
941/640 |
V1927 (f): AAGGGAAGGTTGGAGAGACTC | HQ834416–HQ834431/HQ834432 |
| V1895 (r): CAAGCAGCATTGTACGCCGTC |
||||
| VPS13 | Verticillium albo-atrum (XP_003001174.1) | 665/545 | V1922 (f): GAGACAACGCTTGTTTGCAAGG | HQ834433–HQ834448/HQ834449 |
| V1923 (r): ACATGCGTCGTTCCAAGATCTG |
*Genes: ALR, α-L-rhamnosidase; Bpntase, 3′(2′),5′-bisphosphate nucleotidase; DHC1, Dynein heavy chain; GPHN, Gephyrin, molybdenum cofactor biosynthesis protein; PCS, peroxisomal-coenzyme A synthetase; POB3, FACT complex subunit; SRP72, signal recognition particle protein 72; VPS13, vacuolar protein sorting-associated protein.
†f, forward; r, reverse.
