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Volume 17, Number 8—August 2011

Dispatch

Hepatitis E Virus Genotype 3 in Humans and Swine, Bolivia

Maria Chiara Dell’AmicoComments to Author , Annalisa Cavallo, José Luis Gonzales, Sara Irene Bonelli, Ybar Valda, Angela Pieri, Higinio Segundo, Ramón Ibañez, Antonia Mantella, Filippo Bartalesi, Francesco Tolari, and Alessandro Bartoloni
Author affiliations: Author affiliations: Università degli Studi di Pisa, Pisa, Italy (M.C. Dell’Amico, F. Tolari); Università degli Studi di Firenze, Firenze, Italy (A. Cavallo, S.I. Bonelli, A. Pieri, A. Mantella, F. Bartalesi, A. Bartoloni); Laboratorio de Investigación y Diagnóstico Veterinario, Santa Cruz, Bolivia (J.L. Gonzales, Y. Valda, R. Ibañez); Distrito de Salud Cordillera, Santa Cruz (H. Segundo)

Main Article

Table

Prevalence of antibodies against hepatitis E virus in 236 human serum samples, by patient age, Bartolo and Casas Viejas, Bolivia

Patient age, y No. positive/no. tested (% positive)
95% Confidence interval for total % positive
Bartolo Casas Viejas Total
1–5 2/18 (11) 0/5 (0) 2/23 (8.7) 1.1–28
6–10 0/26 (0) 0/12 (0) 0/38 (0) 0–9.2
11–20 1/35 (2.8) 0/7 (0) 1/42 (2.4) 0–12.6
21–30 1/25 (4) 1/6 (16.7) 2/31 (6.4) 0.8–21.4
31–40 1/19 (5.2) 0/6 (0) 1/25 (4.0) 0.1–20.3
41–50 5/18 (27.8) 0/3 (0) 5/21 (24.0) 8.2–47.1
51–60 1/19 (5.3) 0/10 (0) 1/29 (3.4) 0–17.8
>60
1/12 (8.3)
2/15 (13.3)
3/27 (11.0)
2.3–29.1
Total 12/172 (7) 3/64 (4.7) 15/236 (6.3) 3.6–10.3

Figure. Phylogenetic tree with alignments of 348-bp open reading frame 2 sequences from human and swine samples of hepatitis E virus (HEV), Bartolo and Casas Viejas, Bolivia, compared with sequences of various HEV isolates. The tree was constructed by using the neighbor-joining method and evaluated by using the interior branch test method with MEGA5 software (www.megasoftware.net). Percentage of bootstrap support is shown by values at the branch nodes of the tree. Only nodes with a bootstrap value >60% are labeled; these values are the result of resampling the data 1,000 times. Isolate names are followed by species of origin. Roman numerals indicate genotype. Avian HEV was used as the outgroup. Scale bar indicates nucleotide substitutions per site.

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