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Volume 17, Number 8—August 2011

Research

Novel Human Reovirus Isolated from Children with Acute Necrotizing Encephalopathy

Louise A. Ouattara, Francis Barin, Marie Anne Barthez, Bertrand Bonnaud, Philippe Roingeard, Alain Goudeau, Pierre Castelnau, Guy Vernet, Gláucia Paranhos-BaccalàComments to Author , and Florence Komurian-Pradel
Author affiliations: Author affiliations: Fondation Mérieux, Lyon, France (L.A. Ouattara, G. Vernet, G. Paranhos-Baccalà, F. Komurian-Pradel); Centre Hospitalier Universitaire Bretonneau (F. Barin, P. Roingeard, A. Goudeau); Institut National de la Santé et de la Recherche Médicale U966, Tours, France (F. Barin, P. Roingeard, A. Goudeau); Centre Hospitalier Universitaire, Clocheville, Tours (M.A. Barthez, P. Castelnau); bioMérieux SA, Marcy l’Etoile, France (B. Bonnaud)

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Figure 4

Results of serologic analysis by Western blot of serum specimens from patient 2, a 22-month-old girl with acute necrotizing ancephalopathy. Three serum specimens from patient 2, harvested at 6 (A), 13 (B), and 19 (C) days after onset of symptoms, and a serum specimen from a healthy donor (D) were incubated with reovirus MRV2Tou05–infected and –noninfected BGM cells. I, infected; C, noninfected; M, molecular weight markers (Precision Plus protein standards).

Figure 4. Results of serologic analysis by Western blot of serum specimens from patient 2, a 22-month-old girl with acute necrotizing ancephalopathy. Three serum specimens from patient 2, harvested at 6 (A), 13 (B), and 19 (C) days after onset of symptoms, and a serum specimen from a healthy donor (D) were incubated with reovirus MRV2Tou05–infected and –noninfected BGM cells. I, infected; C, noninfected; M, molecular weight markers (Precision Plus protein standards).

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