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Volume 17, Number 8—August 2011

CME ACTIVITY - Research

Deaths Associated with Human Adenovirus-14p1 Infections, Europe, 2009–2010

Michael J. CarrComments to Author , Adriana E. Kajon, Xiaoyan Lu, Linda Dunford, Paul O’Reilly, Paul Holder, Cillian F. De Gascun, Suzie Coughlan, Jeff Connell, Dean D. Erdman, and William W. Hall
Author affiliations: Author affiliations: National Virus Reference Laboratory–University College Dublin, Dublin, Ireland (M.J. Carr, L. Dunford, P. O’Reilly, P. Holder, C.F. De Gascun, S. Coughlan, J. Connell, W.W. Hall); Lovelace Respiratory Research Institute, Albuquerque, New Mexico, USA (A.E. Kajon); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (X. Lu, D.D. Erdman)

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Figure 2

Comparative restriction enzyme analysis of viral DNA extracted from the prototype human adenovirus (HAdV) 14 de Wit strain and the first detected HAdV-14 case isolated in Dublin, Ireland, November 2009. All odd-numbered lanes (e.g., 1, 3) contain the de Wit strain and all even-numbered lanes (e.g., 2, 4) contain the Dublin 2009 strain, with restriction enzyme digests as follows: lanes 1 and 2 with BamHI; lanes 3 and 4 with BclI; lanes 5 and 6 with BglII; lanes 7 and 8 with BstEII; lanes 9 and 10

Figure 2. Comparative restriction enzyme analysis of viral DNA extracted from the prototype human adenovirus (HAdV) 14 de Wit strain and the first detected HAdV-14 case isolated in Dublin, Ireland, November 2009. All odd-numbered lanes (e.g., 1, 3) contain the de Wit strain and all even-numbered lanes (e.g., 2, 4) contain the Dublin 2009 strain, with restriction enzyme digests as follows: lanes 1 and 2 with BamHI; lanes 3 and 4 with BclI; lanes 5 and 6 with BglII; lanes 7 and 8 with BstEII; lanes 9 and 10 with DraI; lanes 11 and 12 with HindIII; lanes 13 and 14 with PstI; lanes 15 and 16 with SmaI; lanes 17 and 18 with XbaI. Outer lanes are molecular markers (1 Kb +100 bp; BioRad, Hercules, CA, USA).

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