Fatal Neurologic Disease and Abortion in Mare Infected with Lineage 1 West Nile Virus, South Africa
Marietjie Venter , Stacey Human, Stephanie van Niekerk, June Williams, Charmaine van Eeden, and Frank Freeman
Author affiliations: Author affiliations: University of Pretoria, Pretoria, South Africa (M. Venter, S. Human, C. van Eeden, S. van Niekerk, J. Williams); National Institute for Communicable Diseases, Sandringham, Johannesburg, South Africa (M. Venter); Ceres Veterinary Hospital, Western Cape, South Africa (F. Freeman)
Figure 1. Dissociation curve analysis of the diagnostic nested real-time reverse transcription PCR of West Nile virus isolated from a mare and fetus with fatal neurologic disease, South Africa, 2010. Positive control (Kunjin L1b); SAE74/10 (fetus); SAE75/10 (mare). Expected melting peak of lineage 2 = lineage 1b+6°C; lineage 1a = lineage 1b+10°C.
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