Fatal Neurologic Disease and Abortion in Mare Infected with Lineage 1 West Nile Virus, South Africa
Marietjie Venter , Stacey Human, Stephanie van Niekerk, June Williams, Charmaine van Eeden, and Frank Freeman
Author affiliations: Author affiliations: University of Pretoria, Pretoria, South Africa (M. Venter, S. Human, C. van Eeden, S. van Niekerk, J. Williams); National Institute for Communicable Diseases, Sandringham, Johannesburg, South Africa (M. Venter); Ceres Veterinary Hospital, Western Cape, South Africa (F. Freeman)
Figure 2. Maximum-likelihood comparison of a partial section of the E-protein gene (A) and NS5 gene (B) of West Nile virus (WNV) lineage 2 strains isolated in South Africa (SA) in 2010 from a mare with fatal neurologic disease and representative sequences of other WNV lineages from various regions of the world. The lineage 1 strain, SAE75/10, identified in South Africa is indicated by a triangle and arrow. Sequences were aligned with MAFFT version 6 (http://align.bmr.kyushu-u.ac.jp/mafft/software) and edited with BioEdit version 126.96.36.199 (www.mbio.ncsu.edu/bioedit). Maximum-likelihood midpoint rooted trees were drawn by using PHYML (www.atgc-montpellier.fr/phyml) under 100 bootstrap repetitions and the HKY codon position substitution model and drawn to scale with the bars indicating 0.07 nt substitutions. Only bootstrap values >70 are shown. Reference strains used from GenBank that were most closely related to SA L1: PAH001, Tunisia (AY268133); Ast04-2-824A, Russia (DQ374652); Ast02-2-25, Russia (DQ374653); Ast02-2-692, Russia (DQ411031); Ast01-187, Russia (DQ411035); Ge1b/B, Spain (FJ766331); GE-2o/V, Spain (FJ766332); WNV Italy-1998-equine, Italy (AF404757); WNV15803, Italy (FJ483549); and Ita09, Italy (GU011992).
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.