Figure 2. Phylogenetic tree of enterovirus 68 viral protein 1 gene sequences constructed by using a 927-nt sequence corresponding to nt sequence 2355–3281 in strain 37–99, Osaka, Japan, October 2009–October 2010. Tree was constructed by using the neighbor-joining method. Sequences were aligned by using Clustal X version 1.81. (www.clustal.org/). Genetic distances between sequences were calculated by using the Kimura 2-parameter method. Bootstrap values from 1,000 replicates are shown at the nodes. Location and year of collection are shown in parentheses. GenBank accession numbers for strains used in this analysis were Fermon, AY426531; 37–99, EF107098; JPOC10–200, AB601872; JPOC10–290, AB601882; JPOC10–373, AB601873; JPOC10–378, AB601883; JPOC10–396, AB601884; JPOC10–402, AB601874; JPOC10–404, AB601885; JPOC10–412, AB601875; JPOC10–441, AB601876; JPOC10–445, AB601877; JPOC10–471, AB601878; JPOC10–573, AB601879; JPOC10–616, AB601880; JPOC10–618, AB601881; MD02–1, AY426491; TX02–1, AY426495; MO00, AY426493; WI00, AY426494; MN98, AY426497; TX99, AY426498; MD99, AY426499; TX03, AY426500; MN89, AY426489; NY93, AY426490; EV70, D00820; and EV94, DQ916376. Scale bar indicates nucleotide substitutions per site.