Seroprevalence of Trichodysplasia Spinulosa–associated Polyomavirus
Els van der Meijden , Siamaque Kazem, Manda M. Burgers, Rene Janssens, Jan Nico Bouwes Bavinck, Hester de Melker, and Mariet C.W. Feltkamp
Author affiliations: Author affiliations: Leiden University Medical Center, Leiden, the Netherlands (E. van der Meijden, S. Kazem, M.M. Burgers, J.N. Bouwes Bavinck, M.C.W. Feltkamp); Jeroen Bosch Ziekenhuis, ‘s Hertogenbosch, the Netherlands (R. Janssens); National Institute of Public Health and the Environment, Bilthoven, the Netherlands (H. de Melker)
Figure 1. Reproducibility of trichodysplasia spinulosa–polyomavirus (TSV) viral protein 1 (VP1) immunoassay. Seroreactivity against TSV VP1 in 80 renal transplant patients, the Netherlands, was analyzed twice by using the Bio-Plex 100 analyzer (Bio-Rad Laboratories, Hercules, CA, USA). Datasets 1 and 2 were obtained during a 3-month interval by using freshly coupled identical glutathione–casein bead sets coupled independently with the same crude TSV VP1 bacterial extract. Each circle represents 1 serum sample, and the line represents results of linear regression analyses. Correlation coefficient (r2) was determined by using GraphPad Prism software (GraphPad Software Inc., La Jolla, CA, USA). MFI, median fluorescent intensity.
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