Figure 1. Reproducibility of trichodysplasia spinulosa–polyomavirus (TSV) viral protein 1 (VP1) immunoassay. Seroreactivity against TSV VP1 in 80 renal transplant patients, the Netherlands, was analyzed twice by using the Bio-Plex 100 analyzer (Bio-Rad Laboratories, Hercules, CA, USA). Datasets 1 and 2 were obtained during a 3-month interval by using freshly coupled identical glutathione–casein bead sets coupled independently with the same crude TSV VP1 bacterial extract. Each circle represents 1 serum sample, and the line represents results of linear regression analyses. Correlation coefficient (r2) was determined by using GraphPad Prism software (GraphPad Software Inc., La Jolla, CA, USA). MFI, median fluorescent intensity.