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Volume 17, Number 9—September 2011

Research

Classical Bovine Spongiform Encephalopathy by Transmission of H-Type Prion in Homologous Prion Protein Context

Juan-María TorresComments to Author , Olivier Andréoletti, Caroline Lacroux, Irene Prieto, Patricia Lorenzo, Magdalena Larska, Thierry Baron, and Juan-Carlos Espinosa
Author affiliations: Author affiliations: Centro de Investigación en Sanidad Animal, Madrid, Spain (J.-M. Torres, I. Prieto, P. Lorenzo, M. Larska, J.-C. Espinosa); Ecole Nationale Vétérinaire de Toulouse, Toulouse, France (O. Andréoletti, C. Lacroux); Agence Francaise de Sécurité Sanitaire des Aliments, Lyon, France (T. Baron)

Main Article

Figure 2

Western blot analyses of brain protease-resistant prion protein (PrPres) from BSE-H infected mice by using Sha31 monoclonal antibody. A) Mice infected with isolate 07-644 at first passage (lanes 3–7) showing a homogeneous high-type (H-type) PrPres molecular profile; BSE-H isolate 07-644 (lane 1) and a BSE-C isolate (lane 2) were included for comparison. B) Mice infected with isolate 02-2695 at first passage showing either H-type (lanes 1 and 3) or classical BSE–like (C-like) PrPres molecular pro

Figure 2. Western blot analyses of brain protease-resistant prion protein (PrPres) from BSE-H infected mice by using Sha31 monoclonal antibody. A) Mice infected with isolate 07-644 at first passage (lanes 3–7) showing a homogeneous high-type (H-type) PrPres molecular profile; BSE-H isolate 07-644 (lane 1) and a BSE-C isolate (lane 2) were included for comparison. B) Mice infected with isolate 02-2695 at first passage showing either H-type (lanes 1 and 3) or classical BSE–like (C-like) PrPres molecular profile (lanes 2 and 4); BSE-H isolate 02-2695 (lane 6) and a BSE-C isolate (lane 5) were included for comparison. In panel B, a 10-fold equivalent brain tissue mass was loaded for brains from mice showing H-type PrPres molecular profile (lanes 1 and 3) than from those with a C-like PrPres molecular profile (lanes 2 and 4) to obtain equivalent PrPres signals. Values to the left indicate molecular mass in kDa. BSE, bovine spongiform encephalopathy; BSE-H, unglycosylated PrPres that is higher than BSE-C; BSE-C, classical BSE.

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