Classical Bovine Spongiform Encephalopathy by Transmission of H-Type Prion in Homologous Prion Protein Context
Figure 4. Western blot analyses of brain protease-resistant prion protein (PrPres) from BSE-H infected mice by using Saf84 monoclonal antibody. Tg110 mice infected with isolate 02-2695 (lanes 2 and 3) or 45 (lane 4) at first passage showing either high-type (lane 2) or classical BSE–like PrPres molecular profile (lanes 3 and 4). The BSE-H isolate (02–2695) (lane 1) and a BSE-C isolate (lane 5) were included for comparison. Similar quantities of PrPres were loaded in each lane. Values to the left indicate molecular mass in kDa. BSE, bovine spongiform encephalopathy; BSE-H, unglycosylated PrPres that is higher than BSE-C; BSE-C, classical BSE.