Classical Bovine Spongiform Encephalopathy by Transmission of H-Type Prion in Homologous Prion Protein Context
Figure 6. Vacuolar lesion profiles in brains from Tg110 mice inoculated with BSE-H (isolate 02-2695, first passage) showing either H-type PrPres phenotype (black triangles, n = 6 animals) or C-like PrPres phenotype (blue squares, n = 3 animals). Lesion profile in brains from Tg110 inoculated with BSE-C (first passage) is also included for comparison (red circles, n = 6 animals). Lesion scoring was undertaken for 9 areas of gray matter (G) and white matter (W) in mouse brains: dorsal medulla (G1), cerebellar cortex (G2), superior colliculus (G3), hypothalamus (G4) medial thalamus (G5), hippocampus (G6), septum (G7), medial cerebral cortex at the level of the thalamus (G8) and at the level of the septum (G9), cerebellum (W1), mesencephalic tegmentum (W2), and pyramidal tract (W3). Error bars indicate SE. BSE, bovine spongiform encephalopathy; BSE-H, unglycosylated PrPres that is higher than BSE-C; PrPres, protease-resistant prion protein; H-type, high-type Western blot profile of PrPres; C-type, classical-type Western blot profile of PrPres; C-like, classical BSE–like; BSE-C, classical BSE.