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Volume 18, Number 1—January 2012

Research

Assessing Prion Infectivity of Human Urine in Sporadic Creutzfeldt-Jakob Disease

Silvio Notari1, Liuting Qing1, Maurizio Pocchiari, Ayuna Dagdanova, Kristin Hatcher, Arend Dogterom, Jose F. Groisman, Ib Bo Lumholtz, Maria Puopolo, Corinne Lasmezas, Shu G. Chen, Qingzhong Kong, and Pierluigi GambettiComments to Author 
Author affiliations: Case Western Reserve University, Cleveland, Ohio, USA (S. Notari, L. Qing, A. Dagdanova, K. Hatcher, S.G. Chen, Q. Kong, P. Gambetti); Istituto Superiore di Sanità, Rome, Italy (M. Pocchiari, M. Puopolo); Ferring Pharmaceuticals, Hvidore, Denmark (A. Dogterom); Instituto Massone, Buenos Aires, Argentina (J.F. Groisman); BL Consult ApS, Copenhagen, Denmark (I.B. Lumholtz); The Scripps Research Institute, Jupiter, Florida, USA (C. Lasmezas)

Main Article

Table 1

Infectivity titers in microsomal fraction of 4 patients with sCJDMM1*

sCJDMM1 inoculum Brain† dilution Incubation time, d , mean ± SEM Distribution Prion titer, ID50/g tissue†
Patient 1 10−1 256 ± 5 7/7 3.0 × 106
10−2 297 ± 18 4/4
10−3 314 ± 15 5/5
10−4 339 ± 16 5/5
10−5 420, 437 2/5
10−6 >681 0/5
Patient 2 10−4 382 ± 12 5/10 2.2 × 106
Patient 3 10−4 348 ± 12 10/10 1.2 × 107
Patient 4 10−2 267 ± 14 5/5 7.2 × 106

*sCJD, sporadic Creutzfeldt-Jakob disease; ID50, 50% infectious dose.
†Brain tissue equivalent.

Main Article

1These authors contributed equally to this article.

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