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Volume 18, Number 1—January 2012

Research

Serious Invasive Saffold Virus Infections in Children, 2009

Alex Christian Yde NielsenComments to Author , Blenda Böttiger, Jytte Banner, Thomas Hoffmann, and Lars Peter Nielsen
Author affiliations: University of Southern Denmark, Odense, Denmark (A.C.Y. Nielsen); Statens Serum Institut, Copenhagen, Denmark (A.C.Y. Nielsen, B. Böttiger, L.P. Nielsen); University of Aarhus, Aarhus, Denmark (J. Banner); Hvidovre Hospital, Copenhagen (T. Hoffmann)

Main Article

Table

Primers and probe used for diagnostic PCR and primers used to sequence Saffold virus, 2009*

Assay, primer Type Sequence, 5′ → 3′
Diagnostic RT-PCR
Saffold F Forward primer CTA WCA TGC CTC CCC GAT T
Saffold R Reverse primer GYT TAG ACC GGG GGA ACC
Saffold Probe Probe TTT CTG CCC TGC TGG GCG G
VP2 typing
SAFV VP2 OF Outer forward primer GAR ATG ACY AAY CTB TCW GAY AGA GT
SAFV VP2 OR Outer reverse primer CCR TTR AAN ACS GGY TTN AC
SAFV VP2 IF Inner forward primer CGG CYA YAA ACA CKC AAT C
SAFV VP2 IR Inner reverse primer TTD GCR TGY TGN GTC CA
VPI typing, type 2 specific
SAFV-2 VP1 OF1 Outer forward primer 1 GCA GAA AAA GGA AAG GTT GC
SAFV-2 VP1 OF2 Outer forward primer 2 GCA GAA AAA GGC AAA GTT GC
SAFV-2 VP1 OR Outer reverse primer TCT TGG RCA AAA CAC TCT CA
SAFV-2 VP1 IF Inner forward primer CYA TAG CTC TTC CTG AAA AYC A
SAFV-2 VP1 IR Inner reverse primer TGR ACC GAA AAY CTG TCT GC

*RT-PCR, real-time reverse transcription PCR; SAFV, Saffold virus: VP, viral protein.

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