Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

Volume 18, Number 11—November 2012

Research

Sources of Dengue Viruses Imported into Queensland, Australia, 2002–2010

David WarrilowComments to Author , Judith A. Northill, and Alyssa T. Pyke
Author affiliations: Queensland Health Forensic and Scientific Services, Archerfield, Queensland, Australia

Main Article

Table 1

Amplification oligonucleotide primers for DENV genotyping RT-PCRs, Queensland, Australia, 2001–2010*†

DENV assay Forward primer Reverse primer
DENV-1‡ 5′760-AACGTGGATGTCCTCTGAAGG-7803′ 5′1600-CGAGGTCCAAGGCAGTG-15843′
5′1418-GCAACCATAACACCTCAA-14353′ 5′2600-TGGCTGATCGAATTCCACAC-25813′
DENV-2§ 5′-789GAAACATGCCCAGAGAATTGAAACT-8133′ 5′-1920CCCTTCATATTGTACTCTGATAACTATTGTTCC-18883′
5′-1547AAGCTTGGCTGGTGCACAGGCAATGGTT-15743′ 5′-2537GGGGATTCTGGTTGGAACTTGTATTGTTCTGTCC-25043′
DENV-3¶ 5′-291TGGCTAGATGGGGTACCTTC-3103′ or5′-722GCTCCCCATGTCGGCATGGGACTGG-7463′ 5′-1819CATCCCTTTGAGTTTCAATTTGTCCAT-17933′
5′-1685CTAGGATCTCAAGAAGGAGCAATGCA-17103′ 5′-2550ATGGCTGTTGCCACTCTTTTGGGGGA-25253′
DENV-4# 5′-742TGGGATTGGAAACAAGAGCTGAGACATGGATGTC-7753′ 5′-1838CGTGTATGACATTCCCTTGATTCTCAATTTCTCCA-18043′
5′-1569CAATGGTTTTTGGACCTACCTCTACCATGG-15983′ 5′-2539GGGGACTCTGGTTGAAATTTGTACTGTTCTGTCCA-25053′

*DENV, dengue virus; RT-PCR, reverse transcription PCR.
†Numbering is based on DENV-1 strain DENV-1BR/90 (AF226685), DENV-2 strain New Guinea C (AF038403), DENV-3 strain Ba51 (AY858037), DENV-4 strain Dominica 1981 (AF326573). For each DENV serotype, forward primers were paired with the shown respective reverse primers in two separate genotyping RT-PCR assays (A. Pyke, unpub. method).
‡A. Pyke and D. Beasley, unpub. method.
§S. Mei Lok, unpub. data.
¶I. Serafin and A. Pyke, unpub. method.
#C. Howard and A. Pyke, unpub. method.

Main Article

TOP