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Volume 18, Number 12—December 2012

Research

Borrelia, Rickettsia, and Ehrlichia Species in Bat Ticks, France, 2010

Cristina Socolovschi, Tahar Kernif, Didier Raoult, and Philippe ParolaComments to Author 
Author affiliations: Author affiliations: Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes, Marseille, France (C. Socolovschi, T. Kernif, D. Raoult); World Health Organization Collaborative Center for Rickettsial Diseases and Other Arthropod-Borne Bacterial Diseases, Marseille (P. Parola)

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Table

Primers and probes used to detect and confirm the presence of bacteria in Argas vespertilionis ticks collected in 2010 from an attic in France*

Bacterial species Gene target (ref) Primers and probe
Screening
Rickettsia RC0338 (12) 1029-F1: 5′-GAM AAA TGA ATT ATA TAC GCC GCA AA-3′
1029-R1: 5′-ATT ATT KCC AAA TAT TCG TCC TGT AC-3′
1029–1P: 6FAM-CTC AAG ATA AGT ATG AGT TAA ATG TAA A-TAMRA
Borrelia 16S rRNA (13) Bor_16S_3_F: 5′ AGC CTT TAA AGC TTC GCT TGT AG 3′
Bor_16S_3_R: 5′ GCC TCC CGT AGG AGT CTG G 3′
Bor_16S_3_P: 6FAM- CCG GCC TGA GAG GGT GAA CGG TAMRA
Coxiella burnetii IS1111 (14) IS 1111F: 5′-CAA GAA ACG TAT CGC TGT GGC-3′
IS 1111R: 5′-CAC AGA GCC ACC GTA TGA ATC-3′
IS1111P: 6-FAM-CCG AGT TCG AAA CAA TGA GGG CTG-TAMRA
Bartonella ITS (15) Barto ITS3 F: 5′-GAT GCC GGG GAA GGT TTT C-3′
Barto ITS3 R: 5′-GCC TGG GAG GAC TTG AAC CT-3′
Barto ITS3 P: 6 FAM-GCG CGC GCT TGA TAA GCG TG-TAMRA
Ehrlichia 16S rRNA (16) Erli_16S_F: 5′-GGT-ACC-YAC-AGA-AGA-AGT-CC-3′Erli_16S_R: 5′-TAG-CAC-TCA-TCG-TTT-ACA-GC-3′
Confirmation
Rickettsia gltA (17) 409D: 5′-CCT ATG GCT ATT ATG CTT GC-3′1258R: 5′-ATT GCA AAA AGT ACA GTG AAC A-3′
Rickettsia ompA (17) 190–70: 5′-ATG GCG AAT ATT TCT CCA AAA-3′
190–701: 5′- GTT CCG TTAATGGCAGCA TCT-3′
190–180: 5′- GCA GCG ATA ATG CTG AGT A-3′
Borrelia 16S rRNA (13) BF1: 5′-GCT GGC AGT GCG TCT TAA GC-3′BR1: 5′-GCT TCG GGT ATC CTC AAC TC-3′
Borrelia Flagellin (18) Bor1: 5′- TAA TAC GTC AGC CAT AAA TGC-3′
Bor2: 5′- GCT CTT TGA TCA GTT ATC ATT C-3′
C. burnetii IS30 (14) IS30a F: 5′-CGC TGA CCT ACA GAA ATA TGT CC-3′
IS30a R: 5′-GGG GTA AGT AAA TAA TAC CTT CTG G-3′
IS30a P: 6-FAM-CAT GAA GCG ATT TAT CAA TAC GTG TAT GC-TAMRA
Bartonella ITS (15) Urbarto1: 5′-CTT-CGT-TTC-TCT-TTC-TTC-A-3′Urbarto2: 5′-CTT-CTC-TTC-ACA-ATT-TCA-AT-3′
Ehrlichia gltA (19) HER-CS133F: 5′-GGW TTY ATG TCY ACT GCT GC-3′HER-CS778R: 5′-GCN CCM CCA TGM GCT GG-3′

*Ref, reference. Primers in boldface were used for sequencing.

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