Volume 18, Number 12—December 2012
Virulent Avian Infectious Bronchitis Virus, People’s Republic of China
*Thirty-day-old SPF chickens were inoculated intranasally with YN IBV (105 50% egg infectious dose per bird). IBV, infectious bronchitis virus; SPF, specific-pathogen free; RT-PCR, reverse transcription PCR; ND, not detected.
†Ten tissue samples from euthanized birds were randomly collected and examined.
‡RT-PCR was directly performed on collected tissues.
§Tissue samples were collected for re-isolation of the challenge virus using SPF embryos. Data are the number of tissues from which the virus was isolated/number of tissues examined.
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