Leishmania infantum and Human Visceral Leishmaniasis, Argentina
Figure. Case of autochthonous human visceral leishmaniasis in a 44-year-old man, identified by parasitologic diagnosis and molecular detection of the causative species, Salta, Argentina. A) Leishmania amastigotes in a bone marrow smear. N, nucleus; K, kinetoplast; C, cytoplasm (Giemsa stained, original magnification ×1,000). B) Amplification by nested PCR of cytochrome b gene of Leishmania infantum. Arrow indicates amplified fragment of ≈850 bp. Lane B, blank control; lanes 1 and 2, patient bone marrow aspirate samples; lanes 3–6, samples from Leishmania spp.–negative persons; lane P, positive control; lane N, negative control; lane M, 100-bp molecular mass marker.