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Volume 18, Number 3—March 2012

Dispatch

Culturing Stool Specimens for Campylobacter spp., Pennsylvania, USA

Nkuchia M. M’ikanatha, Lisa A. Dettinger, Amanda Perry, Paul Rogers, Stanley M. Reynolds, and Irving NachamkinComments to Author 
Author affiliations: Pennsylvania Department of Health, Harrisburg, Pennsylvania, USA (N.M. M’ikanatha, A. Perry); Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA (N.M. M’ikanatha, I. Nachamkin); Pennsylvania Department of Health, Exton, Pennsylvania, USA (L.A. Dettinger, P. Rogers, S.M. Reynolds)

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Table 2

Campylobacter-specific laboratory practices for 107 Pennsylvania laboratories performing testing of stool specimens, 2009*

Laboratory practice/method No. (%) laboratories
Included in routine testing 104 (97.2)
Special request culture 1 (0.9)
Culture plus antigen testing 1 (0.9)
Perform culture on positive antigen assay 2 (1.9)
Campylobacter antigen testing only 17 (15.8)
Culture broth enrichment usage
Yes (9 Campy-Thio, 1 GNB, 4 unspecified) 14 (13.1)
No 89 (83.2)
No response 4 (3.7)
Length of Incubation, h
24 1 (<1)
48 64 (59.8)
72 33 (30.8)
No response 9 (8.4)
Type of medium used for Campylobacter culture
Campy-BAP 65 (60.7)
CVA 30 (27.8)
Skirrow 2 (1.9)
CSM 2 (1.8)
CCDA or mCCDA 0
Not specified or not cultured 8 (7.5)
Temperature used for culture, °C
37 3 (2.8)
42 96 (89.7)
Not specified 8 (7.5)
Atmosphere used for culture
Microaerobic, 5% O2 96 (89.7)
10% CO2 1 (0.9)
Both 1(0.9)
Not specified 9 (8.4)
Tests used for identification of Campylobacter spp.
Gram stain 96 (89.7)
Oxidase 92 (86)
Catalase 70 (65.4)
Hippurate hydrolysis 51 (47.7)
Naladixic acid/cephalothin disk identification 21 (19.6)
Indoxyl acetate 7 (6.5)
Send to a reference laboratory 6 (5.6)
Other, not specified 33 (30.8)
Performs susceptibility testing 4 (3.7)

*Campy-Thio, Campylobacter thioglycollate broth; GNB, gram-negative broth; Campy-BAP, Campylobacter blood agar plates; CVA, cefoperazone-vancomycin-amphotericin; CSM, charcoal-based selective medium; CCDA, charcoal-cefoperazone-deoxycholate agar; mCCDA, modified CCDA.

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