Ilheus Virus Infection in Human, Bolivia
Figure. Phylogenetic analysis of the nonstructural protein 5 (NS5) gene region of 7 Ilheus virus isolates and a 189-bp nt sequence (FMB 202 Bolivia). Alignments were analyzed by using the neighbor-joining method with the Kimura 2-parameter algorithm in MEGA5 (www.megasoftware.net). Variation rate among sites was modeled with a gamma distribution (shape parameter = 1). Bootstrap confidence limits (from 1,000 replicates) are indicated at each node. Values in boldface below branches were obtained by parsimony analysis. Boldface indicates virus isolated in this study. Rocio virus (GenBank accession no. AF013397) was included as an outgroup on the basis of the phylogram of Kuno and Chang (10). Sequence generated in our study was deposited in GenBank under accession no. JN679229. Scale bar indicates nucleotide substitutions per site.