Figure 2. A) Phylogenetic relationship between Schmallenberg virus and orthobunyaviruses of the Simbu, Bunyamwera, and California serogroups. International Nucleotide Sequence Database Collaboration accession numbers of the sequences in the analysis are indicated in the tree. The neighbor-joining tree is based on the nucleocapsid gene of the small segment (702 nt). Numbers at nodes represent the percentage of 1,000 bootstrap replicates (values <50 are not shown). Scale bar indicates the estimated number of nt substitutions per site. B) Detection of Schmallenberg virus genome in the blood of experimentally infected calves. The highest genome copy number was detected on postinoculation day 4.