TY - JOUR AU - Jenke, Christian AU - Leopold, Shana AU - Weniger, Thomas AU - Rothgänger, Jörg AU - Harmsen, Dag AU - Karch, Helge AU - Mellmann, Alexander T1 - Identification of Intermediate in Evolutionary Model of Enterohemorrhagic Escherichia coli O157 T2 - Emerging Infectious Disease journal PY - 2012 VL - 18 IS - 4 SP - 582 SN - 1080-6059 AB - Highly pathogenic enterohemorrhagic Escherichia coli (EHEC) O157 cause a spectrum of clinical signs that include diarrhea, bloody diarrhea, and hemolytic uremic syndrome. The current evolutionary model of EHEC O157:H7/H– consists of a stepwise evolution scenario proceeding from O55:H7 to a node (hypothetical intermediate) that then branches into sorbitol-fermenting (SF) O157:H– and non-SF (NSF) O157:H7. To identify this hypothetical intermediate, we performed single nucleotide polymorphism analysis by sequencing of 92 randomly distributed backbone genomic regions of 40 O157:H7/H– isolates. Overall, 111 single nucleotide polymorphisms were identified in 75/92 partial open reading frames after sequencing 51,041 nt/strain. The EHEC O157:H7 strain LSU-61 from deer occupied an intermediate position between O55:H7 and both O157 branches (SF and NSF O157), complementing the stepwise evolutionary model of EHEC O157:H7/H–. The animal origin of this intermediate emphasizes the value of nonhuman reservoirs in the clarification of the evolution of human pathogens. KW - EHEC O157 KW - SNP typing KW - single nucleotide polymorphism KW - sorbitol fermentation KW - phylogeny KW - evolution KW - enterohemorrhagic Escherichia coli KW - Escherichia coli KW - E. coli KW - bacteria KW - deer KW - human KW - pathogen DO - 10.3201/eid1804.111414 UR - https://wwwnc.cdc.gov/eid/article/18/4/11-1414_article ER - End of Reference