Volume 18, Number 5—May 2012
Research
Temporal Trends in Bordetella pertussis Populations, Denmark, 1949–2010
Randi Føns Petersen
, Tine Dalby, Ditte Marie Dragsted, Frits Mooi, and Lotte Lambertsen
, Tine Dalby, Ditte Marie Dragsted, Frits Mooi, and Lotte LambertsenTable 1
Primers used for MLVA and MASTdk typing in a study of temporal trends in Bordetella pertussis populations, Denmark, 1949–2009*
| Primer name | Target | Sequence, 5′ → 3′ | Reference |
|---|---|---|---|
| BP-VNTR1-DF | VNTR1 | FAM-CCTGGCGGCGGGAGACGTGGTGGTG | (9) |
| BP-VNTR1-DR | VNTR1 | AAAATTGCGGCATGTGGGCTGACTCTGA | (9) |
| BP-VNTR3-BF | VNTR3 | FAM -GCCTCGGCGAAATTGCTGAAC | (9) |
| BP-VNTR3-BR | VNTR3 | GCGGGCGAGGAAACGCCCGAGACC | (9) |
| BP-VNTR4-CF | VNTR4 | FAM-CGTGCCCTGCGCCTGGACCTG | (9) |
| BP-VNTR4-BR | VNTR4 | GCCGCTGCTCGACGCCAGGGACAA | (9) |
| BP-VNTR5-BF | VNTR5 | FAM-GAAGCCGGCCCACCCGAGCTCCAGGCTCTT | (9) |
| BP-VNTR5-BR | VNTR5 | TGCCGGGTTTCGGCATCTCGATGGGATACG | (9) |
| BP-VNTR6-EF | VNTR6 | FAM-CCAACGGCGGTCTGCTGGGTGGTC | (9) |
| BP-VNTR6-FR | VNTR6 | AGGGCGCTGGTCACGCCACCGAGGAT | (9) |
| BP-PtxP-F | Pertussis toxin promoter | AATCGTCCTGCTCAACCGCC | (10) |
| BP-PtxP-R | Pertussis toxin promoter | GGTATACGGTGGCGGGAGGA | (10) |
| BP-Ptx S1-F2 | Pertussis toxin Subunit 1 | CCCCCTGCCATGGTGTGATC | (11,12) |
| BP-Ptx S1-R2 | Pertussis toxin Subunit 1 | AGAGCGTCTTGCGGTCGATC | (11,12) |
| BP-prn-AF | Pertactin region 1 | GCCAATGTCACGGTCCAA | (11–13) |
| BP-Prn-AR | Pertactin region 1 | GCAAGGTGATCGACAGGG | (11–13) |
| BP-Prn-BF | Pertactin region 2† | AGCTGGGCGGTTCAAGGT | (11–13) |
| BP-Prn-BR/ | Pertactin region 2† | CCGGATTCAGGCGCAACTC | (11–13) |
| BP-tcfAF | Tracheal colonization factor | TTCTTGCGCGTCGTGTCTTC | (9) |
| BP-tcfAR3 | Tracheal colonization factor | GCGGTTGCGGACCTTCAT | (9) |
*MLVA, multilocus variable-number tandem repeat analysis; MASTdk, multiple antigen sequence typing results obtained in Denmark; VNTR, variable-number tandem repeat.
†Pertactin region 2 was sequenced to confirm prn allele type (1 or 7).
