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Volume 18, Number 5—May 2012

Dispatch

Pigs as Natural Hosts of Dientamoeba fragilis Genotypes Found in Humans

Simone M. CacciòComments to Author , Anna Rosa Sannella, Elisabetta Manuali, Fabio Tosini, Marco Sensi, Daniele Crotti, and Edoardo Pozio
Author affiliations: Istituto Superiore di Sanità, Rome, Italy (S.M. Cacciò, A.R. Sannella, F. Tosini, E. Pozio); Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche, Perugia, Italy (E. Manuali, M. Sensi, D. Crotti)

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Table 2

Results of the molecular tests for Dientamoeba fragilis applied to pig and human fecal samples, Italy, 2010–2011*

Sample Farm Microscopy 5.8S
18S
ITS1
Real-time PCR Sequence PCR Sequence PCR Sequence
P21 2 + + ND ND ND
P26 2 + + D. fragilis + D. fragilis† + D. fragilis‡
P27 2 + + D. fragilis ND + unclassified
P37 2 + + ND ND ND
P42 3 + + D. fragilis + D. fragilis† + D. fragilis§
P44 3 + + D. fragilis ND + flagellate
P45 3 + + ND ND ND
P50 3 + + ND ND ND
P52 3 + + D. fragilis ND + unclassified
P54 3 + + D. fragilis ND + flagellate
P56 3 + + D. fragilis + D. fragilis† + unclassified
P59 3 + + D. fragilis + D. fragilis† + unclassified
P60 3 + + ND ND ND
P71 4 + + ND ND ND
P75 4 + + ND ND ND
P91 5 + + ND ND ND
P93 5 + + D. fragilis + D. fragilis† + unclassified
P 97 5 + + D. fragilis + D. fragilis† + flagellate
P111 6 + + ND ND ND
P113 6 + + ND ND ND
P116 6 + + ND ND ND
P122 6 + + D. fragilis ND + flagellate
P131 6 + + ND ND ND
P133 7 + + ND ND ND
Pig 1 1 ND + Trichomitus ND
Pig 2 1 ND ND ND
Pig 3 1 ND + Trichomitus ND
Pig 4 1 ND ND ND
Pig 5 1 ND + Trichomitus ND
Pig 6 1 ND ND ND
Pig 7 1 ND ND ND
Pig 8 1 ND ND ND
Pig 9 1 ND ND ND
Pig 10 1 ND ND ND
DF-P1 6 ND ND ND
DF-P2 6 ND ND ND
DF-P3 6 ND ND ND
DF-P4 6 ND ND ND
H1 2 + D. fragilis + D. fragilis† ND
H2 2 ND ND ND
H3 2 + ND ND ND
H4 2 + ND + D. fragilis† ND
H5 5 + ND ND ND
H6 5 + ND + D. fragilis† ND
H7 3 + ND + D. fragilis† ND
H8 3 + ND ND ND
H9 3 ND ND ND
H10 3 + + ND + D. fragilis† ND
H11 3 + + D. fragilis + D. fragilis† ND
H12 3 + ND ND ND
H13 3 + ND ND ND
H14 3 ND ND ND
H15 8 + + D. fragilis + D. fragilis† + D. fragilis#
H16 8 + ND ND ND
H17 9 + D. fragilis + D. fragilis† + D. fragilis#

*ITS, internal transcribed spacer; ND, not done.
†100% identity to AY730405.
‡100% identity to DQ223443, DQ223447, and DQ223455.
§100% identity to DQ223448 and DQ223453.
¶96% identity to AF124610.
#100% identity to DQ223442, DQ223450, DQ223452, DQ223454, DQ223456, and DQ167586.

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