Volume 18, Number 5—May 2012
Dispatch
Pigs as Natural Hosts of Dientamoeba fragilis Genotypes Found in Humans
Simone M. Cacciò
, Anna Rosa Sannella, Elisabetta Manuali, Fabio Tosini, Marco Sensi, Daniele Crotti, and Edoardo Pozio
, Anna Rosa Sannella, Elisabetta Manuali, Fabio Tosini, Marco Sensi, Daniele Crotti, and Edoardo PozioTable 2
Results of the molecular tests for Dientamoeba fragilis applied to pig and human fecal samples, Italy, 2010–2011*
| Sample | Farm | Microscopy | 5.8S |
18S |
ITS1 |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Real-time PCR | Sequence | PCR | Sequence | PCR | Sequence | |||||
| P21 | 2 | + | + | ND | – | ND | – | ND | ||
| P26 | 2 | + | + | D. fragilis | + | D. fragilis† | + | D. fragilis‡ | ||
| P27 | 2 | + | + | D. fragilis | – | ND | + | unclassified | ||
| P37 | 2 | + | + | ND | – | ND | – | ND | ||
| P42 | 3 | + | + | D. fragilis | + | D. fragilis† | + | D. fragilis§ | ||
| P44 | 3 | + | + | D. fragilis | – | ND | + | flagellate | ||
| P45 | 3 | + | + | ND | – | ND | – | ND | ||
| P50 | 3 | + | + | ND | – | ND | – | ND | ||
| P52 | 3 | + | + | D. fragilis | – | ND | + | unclassified | ||
| P54 | 3 | + | + | D. fragilis | – | ND | + | flagellate | ||
| P56 | 3 | + | + | D. fragilis | + | D. fragilis† | + | unclassified | ||
| P59 | 3 | + | + | D. fragilis | + | D. fragilis† | + | unclassified | ||
| P60 | 3 | + | + | ND | – | ND | – | ND | ||
| P71 | 4 | + | + | ND | – | ND | – | ND | ||
| P75 | 4 | + | + | ND | – | ND | – | ND | ||
| P91 | 5 | + | + | ND | – | ND | – | ND | ||
| P93 | 5 | + | + | D. fragilis | + | D. fragilis† | + | unclassified | ||
| P 97 | 5 | + | + | D. fragilis | + | D. fragilis† | + | flagellate | ||
| P111 | 6 | + | + | ND | – | ND | – | ND | ||
| P113 | 6 | + | + | ND | – | ND | – | ND | ||
| P116 | 6 | + | + | ND | – | ND | – | ND | ||
| P122 | 6 | + | + | D. fragilis | – | ND | + | flagellate | ||
| P131 | 6 | + | + | ND | – | ND | – | ND | ||
| P133 | 7 | + | + | ND | – | ND | – | ND | ||
| Pig 1 | 1 | – | – | ND | + | Trichomitus¶ | – | ND | ||
| Pig 2 | 1 | – | – | ND | – | ND | – | ND | ||
| Pig 3 | 1 | – | – | ND | + | Trichomitus¶ | – | ND | ||
| Pig 4 | 1 | – | – | ND | – | ND | – | ND | ||
| Pig 5 | 1 | – | – | ND | + | Trichomitus¶ | – | ND | ||
| Pig 6 | 1 | – | – | ND | – | ND | – | ND | ||
| Pig 7 | 1 | – | – | ND | – | ND | – | ND | ||
| Pig 8 | 1 | – | – | ND | – | ND | – | ND | ||
| Pig 9 | 1 | – | – | ND | – | ND | – | ND | ||
| Pig 10 | 1 | – | – | ND | – | ND | – | ND | ||
| DF-P1 | 6 | – | – | ND | – | ND | – | ND | ||
| DF-P2 | 6 | – | – | ND | – | ND | – | ND | ||
| DF-P3 | 6 | – | – | ND | – | ND | – | ND | ||
| DF-P4 | 6 | – | – | ND | – | ND | – | ND | ||
| H1 | 2 | – | + | D. fragilis | + | D. fragilis† | – | ND | ||
| H2 | 2 | – | – | ND | – | ND | – | ND | ||
| H3 | 2 | – | + | ND | – | ND | – | ND | ||
| H4 | 2 | – | + | ND | + | D. fragilis† | – | ND | ||
| H5 | 5 | – | + | ND | – | ND | – | ND | ||
| H6 | 5 | – | + | ND | + | D. fragilis† | – | ND | ||
| H7 | 3 | – | + | ND | + | D. fragilis† | – | ND | ||
| H8 | 3 | – | + | ND | – | ND | – | ND | ||
| H9 | 3 | – | – | ND | – | ND | – | ND | ||
| H10 | 3 | + | + | ND | + | D. fragilis† | – | ND | ||
| H11 | 3 | + | + | D. fragilis | + | D. fragilis† | – | ND | ||
| H12 | 3 | – | + | ND | – | ND | – | ND | ||
| H13 | 3 | – | + | ND | – | ND | – | ND | ||
| H14 | 3 | – | – | ND | – | ND | – | ND | ||
| H15 | 8 | + | + | D. fragilis | + | D. fragilis† | + | D. fragilis# | ||
| H16 | 8 | + | – | ND | – | ND | – | ND | ||
| H17 | 9 | – | + | D. fragilis | + | D. fragilis† | + | D. fragilis# | ||
*ITS, internal transcribed spacer; ND, not done.
†100% identity to AY730405.
‡100% identity to DQ223443, DQ223447, and DQ223455.
§100% identity to DQ223448 and DQ223453.
¶96% identity to AF124610.
#100% identity to DQ223442, DQ223450, DQ223452, DQ223454, DQ223456, and DQ167586.
