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Volume 18, Number 6—June 2012

Letter

Human MRSA Isolates with Novel Genetic Homolog, Germany

Suggested citation for this article

To the Editor: Methicillin-resistant Staphylococcus aureus (MRSA) represents a major cause of hospital-, community- and livestock-acquired infections that are increasingly difficult to manage (13). Detection and identification of MRSA by culture and nucleic acid–based methods is challenged by heterogeneous penicillin-binding protein 2a (PBP2a) expression and variability of the staphylococcal cassette chromosome (SCCmec) elements. Recently, a new SCCmec element (XI) carried in bovine and human isolates was described (4,5). This SCCmec element contains a novel mecA homolog, designated mecALGA251, that is not detectable by usual mecA-specific PCR approaches and PBP2a agglutination tests. García-Álvarez et al. reported this novel mecA homolog exhibited 70% identity at DNA level to the mecA gene, and suggested these strains were transmitted from livestock to humans (4).

To search for isolates possessing the novel mecALAG251, we screened S. aureus databases for those entries describing oxacillin/cefoxitin-resistant phenotypes that were negative for mecA by PCR (6) or harbored S. aureus protein A gene (spa) types known to be associated with the occurrence of mecALGA251 (4,5). The databases of the University Hospital Münster contain S. aureus spa typing results of S. aureus isolates obtained from hospital admission screenings and specimens from patients treated at University Hospital Münster. Moreover, they include isolates derived from human and animal subjects, respectively, of 2 cross-border projects between the Netherlands and Germany: MRSA-net EUREGIO Twente/Münsterland and SafeGuard MRSA vet-net (2,7).

The presence of mecALGA251 was verified by using a specific PCR that applied newly designed primers: mecAL1 (5′-AGC TGG CCA TCC CTT TAT TT-3′) and mecAL2 (5′-CTG GCA TAT GGA GAA GAA GAA A-3′), derived from the sequence of S. aureus LGA251 provided by M. Holden (Wellcome Trust Sanger Institute, Hinxton, UK; accession no. FR821779). The sensitivities and specificities of primers were checked by applying S. aureus and other staphylococcal isolates of different clonal backgrounds (8,9). Positive PCR products were sequenced to confirm identification of mecALGA251; the isolates were then characterized by typing the SCCmec region with specific primers for mecR1, mecI, blaZ, ccrA, and ccrB related to type XI SCCmec as described by García-Álvarez et al. (4). Identified isolates were tested for PBP2a by using a latex agglutination assay (Oxoid Deutschland GmbH, Wesel, Germany). We used Etest (bioMérieux SA, Marcy-l'Étoile, France) for antibacterial agent susceptibility testing of β-lactams and other antibacterial agents.

We report on 16 (clinically derived, n = 14; ovine origin, n = 2) oxacillin/cefoxitin-resistant S. aureus isolates possessing the recently described mecALGA251 isolate, but lacking the classical mecA gene currently defining classic MRSA (Table). The isolates belong to spa types t843, t978, t1535, t1773, and t7189. Concurring with the findings in the United Kingdom and Denmark, we found t843 to be the most prevalent spa type. Results of the PBP2a latex agglutination assay were negative for all isolates except for 1 (no. 14), which was indeterminable. García-Álvarez et al. described negative results for all tested isolates (4); Shore et al. reported inconsistent results with this test (5).

According to the Clinical and Laboratory Standards Institute MIC interpretative standards for staphylococci (10), antibacterial agent susceptibility testing revealed resistance to benzylpenicillin and oxacillin/cefoxitin for all isolates. All isolates were shown to produce β-lactamases. Apart from the general categorization of oxacillin/cefoxitin-resistant isolates as resistant to all β-lactams, the MICs of drugs for all isolates included were read as susceptible for imipenem (MIC for 90% of strains tested 0.5 µg/mL) as well as for the anti-MRSA cephalosporin ceftobiprole (MIC for 90% of strains tested 1 µg/mL applying provisional breakpoint <4 µg/mL). A large range of MICs were observed for classic cephalosporins, ranging from those isolates categorized as susceptible (cephalothin, n = 15; cefuroxime, n = 10; ceftriaxone, n = 2; cefepime, n = 9) to those classified as resistant.

We observed relatively low oxacillin/cefoxitin MICs for some of the mecALAG251-positive isolates (MIC 3 µg/mL, n = 1; MIC 4 µg/mL, n = 1; MIC 8 µg/mL, n = 3) compared with the MRSA reference strain ATCC 43300 (MIC 32 µg/mL). All isolates tested were susceptible to all non–β-lactam antibacterial agents, comprising glycopeptides, lipopeptides, fluoroquinolones, macrolides, lincosamides, oxazolidinones, rifampins, streptogramins, glycylcyclines, folate pathway inhibitors, aminoglycosides, and fosfomycin.

Until mecALGA251 is included as a diagnostic target in molecular MRSA detection tests, oxacillin/cefoxitin-resistant isolates determined to be methicillin-susceptible by traditional, culture-based susceptibility testing methods should not be disregarded, even if mecA and/or PBP2a tests fail to detect their targets. Susceptibility patterns of mecALGA251-positive S. aureus isolates revealed low MICs of oxacillin compared with those for MRSA of the classical mecA type. We presume this indicates an altered affinity of β-lactam antibacterial agents to the putative mecALGA251 gene product or a divergent expression of the gene. The choice and the dosage of antibacterial agents applicable for S. aureus infections should be reconsidered in light of this novel mecA homolog in molecular screening and identification tests. Studies are warranted to investigate the prevalence of this novel MRSA entity in and outside of hospitals in the human population and in livestock, its clinical effects, and its response to antibacterial agent therapy.

André Kriegeskorte, Britta Ballhausen, Evgeny A. Idelevich, Robin Köck, Alexander W. Friedrich, Helge Karch, Georg Peters, and Karsten BeckerComments to Author 
Author affiliations: University Hospital Münster, Germany (A. Kriegeskorte, B. Ballhausen, E.A. Idelevich, R. Köck, H. Karch, G. Peters, K. Becker); University Medical Center Groningen, Groningen, the Netherlands (A.W. Friedrich)

Acknowledgments

We sincerely thank B. Grünastel, D. Kuhn, E. Leidig, M. Schulte, and M. Tigges for excellent technical assistance.

This work was supported by grants to R.K., A.F., G.P., and K.B. (01KI1014A) within the MedVet-Staph project from the Bundesministerium für Bildung und Forschung, Germany; grants to G.P., and K.B. (BMG 090304) from the Bundesministerium für Gesundheit, Germany; and grants to R.K., A.F., G.P., and K.B. from the INTERREG IVa programme of the European Union (SafeGuard MRSA vet-net, no. III-2-03=025).

References

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  2. Köck R, Harlizius J, Bressan N, Laerberg R, Wieler LH, Witte W, Prevalence and molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) among pigs on German farms and import of livestock-related MRSA into hospitals. Eur J Clin Microbiol Infect Dis. 2009;28:137582. DOIPubMed
  3. Klein E, Smith DL, Laxminarayan R. Community-associated methicillin-resistant Staphylococcus aureus in outpatients, United States, 1999–2006. Emerg Infect Dis. 2009;15:192530. DOIPubMed
  4. García-Álvarez L, Holden MT, Lindsay H, Webb CR, Brown DF, Curran MD, Meticillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study. Lancet Infect Dis. 2011;11:595603. DOIPubMed
  5. Shore AC, Deasy EC, Slickers P, Brennan G, O'Connell B, Monecke S, Detection of staphylococcal cassette chromosome mec type XI encoding highly divergent mecA, mecI, mecR1, blaZ and ccr genes in human clinical clonal complex 130 methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2011;55:376573. DOIPubMed
  6. Murakami K, Minamide W, Wada K, Nakamura E, Teraoka H, Watanabe S. Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. J Clin Microbiol. 1991;29:22404.PubMed
  7. Friedrich AW, Daniels-Haardt I, Köck R, Verhoeven F, Mellmann A, Harmsen D, EUREGIO MRSA-net Twente/Münsterland–a Dutch-German cross-border network for the prevention and control of infections caused by methicillin-resistant Staphylococcus aureus. Euro Surveill. 2008;13 [cited 2012 Mar 30]. http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=18965.
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  10. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing: twenty-first informational supplement (M100–S21). Wayne (PA): The Institute; 2011.

Table

Suggested citation for this article: Kriegeskorte A, Ballhausen B, Idelevich E A, Köck R, Friedrich AW, Karch H, et al. Human MRSA isolates with novel genetic homolog, Germany [letter]. Emerg Infect Dis [serial on the Internet]. 2012 Jun [date cited]. http://dx.doi.org/10.3201/eid1806.110910

DOI: 10.3201/eid1806.110910

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Table of Contents – Volume 18, Number 6—June 2012

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