Macrolide-Resistant Bordetella pertussis Infection in Newborn Girl, France
Sophie Guillot, Ghislaine Descours, Yves Gillet, Jérome Etienne, Daniel Floret, and Nicole Guiso
Author affiliations: Institut Pasteur, Paris, France (S. Guillot, N. Guiso); Hospices Civils de Lyon, Bron, France (G. Descours, J. Etienne, Y. Gillet, D. Floret); University Claude Bernard, Lyon, France (G. Descours, J. Etienne, D. Floret)
Figure. . . Screening for the A2047G mutation by PCR–restriction fragment length polymorphism analysis. The 521-bp fragment of the 23S rDNA gene amplified by PCR from the Bordetalla pertussis clinical isolates (FR4229, FR4930, and FR4991) and controls (A228 and Tohama I) was digested with the endonuclease BbsI. Lanes 1 and 7, M, 100-bp ladder (SM0321; Fermentas, St. Leon-Rot, Germany); lane 2, B. pertussis FR4929; lane 3, B. pertussis FR4930; lane 4, B. pertussis FR4991; lane 5, control B. pertussis A228 (erythromycin resistant, heterozygous); lane 6, control B. pertussis Tohama (erythromycin susceptible).
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