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Volume 18, Number 6—June 2012

Dispatch

Macrolide-Resistant Bordetella pertussis Infection in Newborn Girl, France

Sophie Guillot, Ghislaine Descours, Yves Gillet, Jérome Etienne, Daniel Floret, and Nicole GuisoComments to Author 
Author affiliations: Institut Pasteur, Paris, France (S. Guillot, N. Guiso); Hospices Civils de Lyon, Bron, France (G. Descours, J. Etienne, Y. Gillet, D. Floret); University Claude Bernard, Lyon, France (G. Descours, J. Etienne, D. Floret)

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Figure

Screening for the A2047G mutation by PCR–restriction fragment length polymorphism analysis. The 521-bp fragment of the 23S rDNA gene amplified by PCR from the Bordetalla pertussis clinical isolates (FR4229, FR4930, and FR4991) and controls (A228 and Tohama I) was digested with the endonuclease BbsI. Lanes 1 and 7, M, 100-bp ladder (SM0321; Fermentas, St. Leon-Rot, Germany); lane 2, B. pertussis FR4929; lane 3, B. pertussis FR4930; lane 4, B. pertussis FR4991; lane 5, control B. pertussis A228 (e

Figure. . . Screening for the A2047G mutation by PCR–restriction fragment length polymorphism analysis. The 521-bp fragment of the 23S rDNA gene amplified by PCR from the Bordetalla pertussis clinical isolates (FR4229, FR4930, and FR4991) and controls (A228 and Tohama I) was digested with the endonuclease BbsI. Lanes 1 and 7, M, 100-bp ladder (SM0321; Fermentas, St. Leon-Rot, Germany); lane 2, B. pertussis FR4929; lane 3, B. pertussis FR4930; lane 4, B. pertussis FR4991; lane 5, control B. pertussis A228 (erythromycin resistant, heterozygous); lane 6, control B. pertussis Tohama (erythromycin susceptible).

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