Figure 1. . . . Three regions of Ecuador where guinea pig serum samples were obtained: Cuenca, Guayaquil, and Manabi. The country is bordered by Colombia to the north, Peru to the east and south, and the Pacific Ocean to the west. Cuenca is located in the Andes; the average annual mean temperature is 14.7°C, and the average annual relative humidity is 85%. Guayaquil is located at the head of the Gulf of Guayaquil; the mean temperature is 26.1°C, and relative humidity is 74%. The Manabí region is located on the Pacific Ocean coast; the mean temperature is 25.9°C, and relative humidity is 79%.