Figure 2. . . . Results of Western blot analyses of pooled serum samples from adult guinea pigs in Ecuador. The guinea pigs were obtained from farms in Cuenca, where they had been raised as livestock, or from live animal markets in Guayaquil and Manabí. The results show different influenza antigens: recombinant hemagglutinin (rHA) A/New Caledonia/20/1999 (A); rHA A/Wisconsin/67/2005 (B); rHA A/Vietnam/1203/2004 (C); recombinant nucleoprotein A/Puerto Rico/08/1934 (D); and B virus (whole virus B/Yamagata/16/1988) (E). Molecular weights are shown at left. (+), serum from guinea pigs infected in the laboratory with influenza virus; (−), serum from naive guinea pig; lanes 1–4, pooled serum samples from Cuenca; lanes 5–6, pooled serum samples from Guayaquil; lanes 7–8, pooled serum samples from Manabí; S, secondary antibody only.