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Volume 18, Number 7—July 2012

Dispatch

Potential International Spread of Multidrug-Resistant Invasive Salmonella enterica Serovar Enteritidis

Irene RodríguezComments to Author , M. Rosario Rodicio, Beatriz Guerra, and Katie L. Hopkins
Author affiliations: Federal Institute for Risk Assessment, Berlin, Germany (I. Rodríguez, B. Guerra); Universidad de Oviedo, Oviedo, Spain (M.R. Rodicio); and Health Protection Agency, London, UK (K.L. Hopkins)

Main Article

Table 2

Characteristics of Salmonella enterica serovar Enteritidis isolates harboring pUO-SeVR1-like plasmids, 2005–2010, UK*

Isolate no. Phage type Resistance phenotype/ genotype Class 1 integron† pSEV genes‡ MLVA MLST VR plasmid, kb
CNM4839/03 PT 14b AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5,srgB, srgC, pefA, pefB, pefC, pefD 2-12-9-4-4-3-NA-8-8 ST11 100
H051860415 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, srgB, srgC, pefA, pefB, pefC, pefD 2-13-9-4-4-3-NA-8-8 ST1479 95
H070360201 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, ΔsrgA, srgB, srgC, pefA, pefB 2-15-9-4-4-3-NA-8-8 ST1479 88
H070420137 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, ΔsrgA, srgB, srgC, pefA, pefB 2-15-9-4-4-3-NA-8-8 ND 88
H073180204 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, srgB, srgC, pefA, pefB, pefC, pefD 2-13-9-4-4-3-NA-8-8 ND 92
H091340084 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, ΔsrgA, srgB, srgC, pefA, pefB, pefC, pefD 2-9-9-4-4-3-NA-8-8 ST11 90
H091800482 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, srgB, srgC, pefA, pefB, pefC, pefD 2-13-9-4-4-3-NA-8-8 ND 92
H095100307 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, ΔsrgA, srgB, srgC, pefA, pefB, pefC, pefD 2-13-9-4-4-3-NA-8-8 ND 95
H100240198 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, ΔsrgA, srgB, srgC, pefA, pefB, pefC, pefD 2-13-9-4-4-3-NA-8-8 ND 95
H101700366 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, ΔsrgA, srgB, srgC, pefA, pefB, pefC, pefD 2-13-9-4-4-3-NA-8-8 ND 95

*pSEV, serovar-specific V plasmid of S.enterica serovar Enteritidis; MLVA, multilocus variable number tandem repeat analysis; MLST, multilocus sequence typing; VR, virulence-resistance; PT, phage type; NA, no amplification from this locus; AMP, ampicillin; CHL, chloramphenicol; STR streptomycin; SUL, sulfonamides; TET, tetracycline; TMP, trimethoprim; ST, sequence type; ND, not done.
†Size of the variable region amplified with the 5′CS and 3′CS primers (2).
‡All plasmids were positive for IncFIIA, IncFIB and the par locus. Two new primer pairs were devised for detection of srgA: srgAB-Fw1/Rv1 (5′-CGCCTTCCGTGTATGTCC/GCGAGTCACTCACCGACAG-3′) and srgAB-Fw2/Rv2 (5′-GTTGCACAGGAGTGGGAGTC/GTCCGGGTTCCATGTCAG-3′). The forward primers anneal at different positions within srgA; the reverse primers anneal at different positions within srgB.

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