Culicoids as Vectors of Schmallenberg Virus
Figure. . . RNA extracted from pools of Culicoides obsoletus group midges was tested in 1-step reverse transcription quantitative PCRs (RT-qPCRs) for the Schmallenberg virus large segment, and the products were analyzed by agarose gel electrophoresis. Lanes 1–8, C. obsoletus group midge pools 1–8; lanes 9–10; negative and positive controls, respectively. Numbers below lanes are cycle threshold values from RT-qPCRs; –, no value. M, size marker. Amplicons (145 bp) from positive pools were extracted and sequenced.