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Volume 18, Number 7—July 2012

Letter

Culicoids as Vectors of Schmallenberg Virus

Lasse Dam Rasmussen, Birgit Kristensen, Carsten Kirkeby, Thomas Bruun Rasmussen, Graham J. Belsham, René Bødker, and Anette BøtnerComments to Author 
Author affiliations: Technical University of Denmark, Kalvehave, Denmark (L.D. Rasmussen, T.B. Rasmussen, G.J. Belsham, A. Bøtner); Technical University of Denmark, Copenhagen, Denmark (B. Kristensen, C. Kirkeby, R. Bødker)

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Figure

RNA extracted from pools of Culicoides obsoletus group midges was tested in 1-step reverse transcription quantitative PCRs (RT-qPCRs) for the Schmallenberg virus L segment, and the products were analyzed by agarose gel electrophoresis. Lanes 1–8, C. obsoletus group midge pools 1–8; lanes 9–10; negative and positive controls, respectively. Numbers below lanes are cycle threshold values from RT-qPCRs; –, no value. M, size marker. Amplicons (145 bp) from positive pools were extracted and sequenced.

Figure. . . RNA extracted from pools of Culicoides obsoletus group midges was tested in 1-step reverse transcription quantitative PCRs (RT-qPCRs) for the Schmallenberg virus large segment, and the products were analyzed by agarose gel electrophoresis. Lanes 1–8, C. obsoletus group midge pools 1–8; lanes 9–10; negative and positive controls, respectively. Numbers below lanes are cycle threshold values from RT-qPCRs; –, no value. M, size marker. Amplicons (145 bp) from positive pools were extracted and sequenced.

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