Epidemic Clostridium difficile Ribotype 027 in Chile
Cristian Hernández-Rocha1, Jonathan Barra-Carrasco1, Marjorie Pizarro-Guajardo, Patricio Ibáñez, Susan M. Bueno, Mahfuzur R. Sarker, Ana María Guzman, Manuel Álvarez-Lobos, and Daniel Paredes-Sabja
Author affiliations: Pontificia Universidad Católica de Chile, Santiago, Chile (C. Hernández-Rocha, P. Ibáñez, S.M. Bueno, A.M. Guzman, M. Álvarez-Lobos); Universidad Andrés Bello, Santiago (J. Barra-Carrasco, M. Pizarro-Guajardo, D. Pareses-Sabja); and Oregon State University, Corvallis, Oregon, USA (M.R. Sarker, D. Paredes-Sabja)
Figure. . Results of PCR ribotyping of Clostridium difficile 027 strains from Chile. M indicates the 100-bp DNA ladder; lane 2, R20291; lane 2, PUC47; lane 3, PUC51. A) PCR ribotyping of C. difficile isolates. PCR results show that that the band pattern of the ribosomal intergenic regions of strains PUC47 and PUC51 are similar to those of the reference (epidemic) strain R20291. B) Cluster analysis of strains PUC47, PUC51, and the epidemic strain 027 R20291 shows >99% similarity and that they belong to the same epidemic clade. Scale bar indicates percent identity.
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