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Volume 18, Number 9—September 2012

Letter

Entamoeba bangladeshi nov. sp., Bangladesh

Tricia L. Royer1, Carol Gilchrist1, Mamun Kabir1, Tuhinur Arju, Katherine S. Ralston, Rashidul Haque, C. Graham Clark, and William A. PetriComments to Author 
Author affiliations: University of Virginia School of Medicine, Charlottesville, Virginia, USA (T.L. Royer, C. Gilchrist, K.S. Ralston, W.A. Petri, Jr.); International Centre for Diarrheal Disease Research, Dhaka, Bangladesh (M. Kabir, T. Arju, R. Haque); and London School of Hygiene and Tropical Medicine, London, UK (C.G. Clark)

Main Article

Table

Oligonucleotide primers used for screening and sequencing of Entamoeba bangladeshi nov. sp., Bangladesh*

Target organism Primer name Primer sequence, 5′ → 3′ Reference
Broad specificity Entamoeba sp. Entagen-F ACT TCA GGG GGA GTA TGG TCA C (7)
Entagen-R CAA GAT GTC TAA GGG CAT CAC AG (7)
E. histolytica Eh-F AAC AGT AAT AGT TTC TTT GGT TAG TAA AA (9)
Eh-R CTT AGA ATG TCA TTT CTC AAT TCA T (9)
Eh-YYT Probe YYT-ATT AGT ACA AAA TGG CCA ATT CAT TCA-Dark Quencher (9)
E. moshkovskii Em-1 CTC TTC ACG GGG AGT GCG (8)
Em-2 TCG TTA GTT TCA TTA CCT (8)
nEm-1 GAA TAA GGA TGG TAT GAC (8)
nEm-2 AAG TGG AGT TAA CCA CCT (8)
E. dispar E-1 TTT GTA TTA GTA CAA A (10)
E-2 GTA [A/G]TA TTG ATA TAC T (10)
Ed-1 AGT GGC CAA TTT ATG TAA GT (10)
Ed-2 TTT AGA AAC AAT GTT TCT TC (10)

*Boldface indicates the probe fluorophore and quencher.

Main Article

1These authors contributed equally to this article.

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