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Volume 18, Number 9—September 2012

Dispatch

Rapid Detection of Carbapenemase-producing Enterobacteriaceae

Patrice NordmannComments to Author , Laurent Poirel, and Laurent Dortet
Author affiliations: Faculté de Médecine Paris Sud, Le Kremlin-Bicêtre, France (P. Nordmann, L. Poirel, L. Dortet); and Institut National de la Santé et de la Recherche Médicale, Paris, France (P. Nordmann, L. Poirel, L. Dortet)

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Table 1

Carbapenemase-producing clinical enterobacterial isolates subjected to the Carba NP test*

Ambler class, carbapenemase type Species β-Lactamase No. MIC range, mg/L
Carba NP test result
IMP ERT MER
Class A
KPC-type
Klebsiella pneumoniae KPC-2 27 0.5–>32 4–>32 1–>32 +
KPC-3 3 0.5–8 4–>32 1–8 +
Klebsiella ozaenae KPC-3 1 >32 >32 2 +
Escherichia coli KPC-2 5 0.5–4 0.5>32 0.5–2 +
Enterobacter cloacae KPC-2 7 1–24 1.5–32 0.75–16 +
Enterobacter aerogenes KPC-2 1 8 >32 8 +
Citrobacter freundii KPC-2 2 8–>32 1.5–>32 1.5–3 +
Serratia marcescens KPC-2 2 >32 >32 >32 +
Salmonella spp.
KPC-2
1
4
1
0.25
+
NMC-A
E. cloacae
NMC-A
1
16
>32
16
+
SME-type
S. marcescens
SME-1 1 32 4 12 +
SME-2
1
32
4
12
+
GES-type
E. cloacae
GES-5
1
>32
>32
>32
+
IMI-type
Enterobacter asburiae
IMI-2
1
>32
>32
>32
+
Class B
NDM-type K. pneumoniae NDM-1 16 0.5–>32 2–>32 1–>32 +
NDM-4 1 >32 >32 >32 +
E. coli NDM-1 7 1–16 3–>32 1–16 +
E. cloacae NDM-1 1 2 16 2 +
C. freundii NDM-1 1 >32 >32 >32 +
Providencia stuartii NDM-1 1 12 0.38 1.5 +
Proteus rettgeri
NDM-1
1
3
0.5
1.5
+
VIM-type
K. pneumoniae VIM-1 15 0.5–>32 0.5–>32 0.38–>32 +
VIM-19 1 8 16 4 +
E. coli VIM-1 2 1.5–3 0.38–1.5 0.5–1 +
VIM-2 2 2–4 0.5–1.5 0.38–0.5 +
VIM-19 1 8 16 4 +
E. cloacae VIM-1 4 1–>32 0.38 to >32 0.5–>32 +
S. marcescens
VIM-2
1
>32
>32
>32
+
IMP-type
K. pneumoniae IMP-1 5 0.5–8 2–4 1–8 +
IMP-8 2 0.5–1 0.5–1 0.5 +
E. coli IMP-1 2 0.5 3–4 0.5–1 +
IMP-8 1 6 8 3 +
E. cloacae IMP-1 12 8–>32 >32 2–>32 +
IMP-8 2 0.75–1.5 0.5–1 0.5–1 +
S. marcescens IMP-1 2 8–>32 >32 2–>32 +

IMP-11
1
8
>32
2
+
Class D
OXA-48 type K. pneumoniae OXA-48 15 0.38–>32 0.38–>32 0.38–>32 +
OXA-181 2 0.5-1 2–4 0.5–1 +
E. coli OXA-48 6 0.38–3 0.5–16 0.12–1 +
E. cloacae OXA-48 3 0.5–1 0.5–16 0.5–1.5 +
P. rettgeri OXA-181 1 8 1 2 +

*IMP, imipenem; ERT, ertapenem; MER, meropenem; KPC, K. pneumoniae carbapenemase; NMC-A, non–metallo-enzyme carbapenemase; SME, S. marscescens enzyme; GES, Guiana extended-spectrum β-lactamase; IMI, imipenem-hydrolysing β-lactamase; NDM, New Delhi metallo-β-lactamase; VIM, Verona integron–encoded metallo-β-lactamase; IMP, imipenemase; OXA-48, oxacillinase-48.

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