Novel Polyomavirus associated with Brain Tumors in Free-Ranging Raccoons, Western United States
Figure 4. . Partial genome sequences (ranging from 2,998 bp in raccoon polyomavirus 6 [RacPyV6] to 4,667 bp in RacPyV9) were obtained from 4 raccoons that either had undergone prolonged storage (RacPyV9) or were available only as formalin-fixed, paraffin-embedded tissue (dashed lines, RacPyVs 1, 6, and 7). Gaps in the sequences correspond to regions where amplification reactions failed. The genomes of RacPyVs 2, 3, 4, 5, 8, and 10 were sequenced in their entirety by using a primer walking method to complete the RacPyV circular genome. Open circles represent noncoding mutations; the closed circle represents a coding mutation. Horizontal bars indicate deletions. LT-Ag, large T-antigen; VP, viral protein; NCRR, noncoding regulatory region.