Spontaneous Generation of Infectious Prion Disease in Transgenic Mice
Figure 1. . . Immunoblots of brain extracts from hemizygous 113LBoPrP-Tg037+/− 113LBoPrP-Tg009+/− mouse lines compared with those of cow brain extract and BoPrP-Tg110 mouse brain extract. Brain homogenates were analyzed by Western blotting with monoclonal antibody 2A11 (30). Lane 1, 113LBoPrPTg-009; lane 2, 113LBoPrPTg-037; lane 3, Cow; lane 4, BoPrPTg-110. Equivalent amounts of total protein were loaded into each lane. 113L, leucine substitution at codon 113; BoPrP, bovine prion protein. Values on the right are molecular masses in kilodaltons.