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Volume 19, Number 12—December 2013

Dispatch

Concomitant Human Infections with 2 Cowpox Virus Strains in Related Cases, France, 2011

Corinne Ducournau, Audrey Ferrier-Rembert, Olivier Ferraris, Aurélie Joffre, Anne-Laure Favier, Olivier Flusin, Dieter Van Cauteren, Kaci Kecir, Brigitte Auburtin, Serge Védy, Maël Bessaud, and Christophe N. PeyrefitteComments to Author 
Author affiliations: French Army Biomedical Research Institut, Grenoble and Lyon, France (C. Ducournau, A. Ferrier-Rembert, O. Ferraris, A. Joffre, A.-L. Favier, O. Flusin, C.N. Peyrefitte); National Reference Center for Orthopoxviruses, Grenoble (C. Ducournau, O. Ferraris, C.N. Peyrefitte); French Institute for Public Health Surveillance, Lyon (D. Van Cauteren); Regional Hospital of Epinal, Epinal, France (K. Kecir, B. Auburtin); Legouest Military Hospital, Metz, France (S. Védy); UMR190 Aix Marseille University EHESP French School of Public Health, Marseille, France (M. Bessaud)

Main Article

Figure 2

Schematic representation of cowpox virus (CPXV) GRI genome (A) and phylogenetic relationships between 4 genomic regions of CPXV isolates collected in France during 2011 and other CPXVs: C18L (B), 14-kDa (C), G1L (D), hemagglutinin (E). The sequenced regions are shaded in black in panel A. Nucleotide sequences were aligned by using CLC Main Workbench 6.0 software (CLC Bio,Aarhus, Denmark). Neighbor-joining phylograms were constructed in MEGA4 (www.megasoftware.net) by using the maximum composite

Figure 2. . Schematic representation of cowpox virus (CPXV) GRI genome (A) and phylogenetic relationships between 4 genomic regions of CPXV isolates collected in France during 2011 and other CPXVs: C18L (B), 14-kDa (C), G1L (D), hemagglutinin (E). The sequenced regions are shaded in black in panel A. Nucleotide sequences were aligned by using CLC Main Workbench 6.0 software (CLC Bio,Aarhus, Denmark). Neighbor-joining phylograms were constructed in MEGA4 (www.megasoftware.net) by using the maximum composite likelihood method; the robustness of the resulting trees was assessed with 1,000 bootstrap replicates. The length of the branches is proportional to the number of base substitutions per site. The percentage bootstrap values are indicated if >70. Circles indicate the 2011 isolates from France; for other isolates, the year and country of isolation are indicated. Scale bars indicate nucleotide substitutions per site.

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