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Volume 19, Number 2—February 2013

Dispatch

Ebola Virus Antibodies in Fruit Bats, Bangladesh

Kevin J. OlivalComments to Author , Ariful Islam, Meng Yu, Simon J. Anthony, Jonathan H. Epstein, Shahneaz Ali Khan, Salah Uddin Khan, Gary Crameri, Lin-Fa Wang, W. Ian Lipkin, Stephen P. Luby, and Peter Daszak
Author affiliations: Author affiliations: EcoHealth Alliance, New York, New York, USA (K.J. Olival, A. Islam, S.J. Anthony, J.H. Epstein, P. Daszak); Commonwealth Scientific and Industrial Research Organisation Australian Animal Health Laboratory, Geelong, Victoria, Australia (M. Yu, G. Crameri, L.-F. Wang); Duke–National University of Singapore Graduate Medical School, Singapore (L.-F. Wang); Chittagong Veterinary and Animal Sciences University, Chittagong, Bangladesh (S.A. Khan); International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh (S.U. Khan, S.P. Luby); Columbia University, New York, New York, USA (W.I. Lipkin)

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Table 1

Ebola virus serologic assay results for bats, Bangladesh, 2010–2011*

Year, specimen no. Age of bat Sex of bat Species or control ELISA OD (endpoint titration)
Western blot
R + Z R Z R Z
2010
Rab691/d0 ND ND Negative control 0.138 0.116 0.097
April 2010–001 A F Negative control (Rousettus leschenaultii) 0.215 0.117 (50) 0.058 (50)
April 2010–002 A F Negative control (R. leschenaultii) 0.092 0.096 0.059
Rab691/EboV-N ND ND Positive control 2.303 1.72 1.23 ++ ++
Monkey/EboV ND ND Positive control 1.753 0.676 0.445 NT NT
April 2010–042 A M R. leschenaultii 1.512 0.511 (400) 0.07 (50) + +
April 2010–057 A M R. leschenaultii 0.684 0.072 (50) 0.477 (800) + ++
66 additional negative
ND
ND
R. leschenaultii
<0.60



NT
NT
2011
Rab691/d0 ND ND Negative control 0.165 0.116 0.145
SB0311–115 A F Negative control (Megaderma lyra) 0.515 0.074 0.083
SB0311–117 A F Negative control (M. lyra) 0.775 0.075 0.072
Rab691/ REboV-N ND ND Positive control 1.598 1.123 1.106 ++ ++
SB0311–001 A M R. leschenaultii 0.494 0.213 (50) 0.538 (100) + +
SB0311–004 A M R. leschenaultii 0.557 0.152 (50) 0.497 (100) + +
SB0311–059 A M R. leschenaultii 0.757 0.079 (50) 0.816 (400) ++
SB0311–016 A F R. leschenaultii 0.542 0.182 (100) 0.367 (400) NT NT
67 additional negative ND ND R. leschenaultii <0.60 NT NT NT NT
55 additional negative ND ND M. lyra <0.775 NT NT NT NT
75 negative ND ND Cynopterus sp. <0.595 NT NT NT NT
1 negative A M Macroglossus sobrinus <0.256 NT NT NT NT

*Values in boldface are positive results. OD, optical density; R + Z, ELISA using a 1:1 mixture of recombinant nucleoproteins of Reston and Zaire Ebola viruses; R, Reston Ebola virus ELISA; Z, Zaire Ebola virus ELISA; ND, not determined;; A, adult; –, negative; ++, strongly positive; NT, not tested; +, positive.

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