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Volume 19, Number 2—February 2013

Dispatch

Human Granulocytic Anaplasmosis, Japan

Norio Ohashi1Comments to Author , Gaowa1, Wuritu, Fumihiko Kawamori, Dongxing Wu, Yuko Yoshikawa, Seizou Chiya, Kazutoshi Fukunaga, Toyohiko Funato, Masaaki Shiojiri2, Hideki Nakajima3, Yoshiji Hamauzu, Ai Takano, Hiroki Kawabata, Shuji Ando, and Toshio Kishimoto

Author affiliations: University of Shizuoka and Global Center of Excellence Program, Shizuoka City, Japan (N. Ohashi, Gaowa, Wuritu, F. Kawamori, D. Wu, Y. Yoshikawa); Kochi Institute of Health, Kochi City, Japan (S. Chiya, K. Fukunaga); Shizuoka Institute of Environment and Hygiene, Shizuoka City (F. Kawamori); Muroto Hospital, Muroto, Japan (T. Funato); Kochi Prefectural Aki Hospital, Aki City, Japan (M. Shiojiri, H. Nakajima); Chu-gei Clinic, Aki District, Japan (Y. Hamauzu); National Institute of Infectious Diseases, Tokyo, Japan (A. Takano, H. Kawabata, S. Ando); Okayama Prefectural Institute for Environmental Science and Public Health, Okayama, Japan (T. Kishimoto)

Main Article

Figure 2

Western blot analyses, using recombinant P44-1 protein (rP44-1) and Anaplasma phagocytophilum–infected THP-1 cells as antigens, of serum samples from 2 men, case-patients 1 and 2, who had A. phagocytophilum infection, Kochi Prefecture, Japan. The Escherichia coli, which produced rP44-1, was kindly provided by Yasuko Rikihisa (Ohio State University, Columbus, OH, USA). The rP44-1 and the rabbit hyperimmune serum (positive control serum) were prepared as described (11,12). Results for a negative c

Figure 2. . . Western blot analyses, using recombinant P44-1 protein (rP44-1) and Anaplasma phagocytophilum–infected THP-1 cells as antigens, of serum samples from 2 men, case-patients 1 and 2, who had A. phagocytophilum infection, Kochi Prefecture, Japan. The Escherichia coli, which produced rP44-1, was kindly provided by Yasuko Rikihisa (Ohio State University, Columbus, OH, USA). The rP44-1 and the rabbit hyperimmune serum (positive control serum) were prepared as described (11,12). Results for a negative control (human serum sample) are included. The primary human serum samples tested were diluted 200- to 400-fold; rabbit serum sample (positive control) was diluted 2,000-fold. The goat antihuman IgG and IgM alkaline phosphatase conjugates (Life Technologies, Grand Island, NY, USA) were used as secondary antibodies. Days represent days after symptom onset. rP, rP44-1 antigen; In, infected THP-1; Un, uninfected THP-1 cells.

Main Article

1These authors contributed equally to this article.

2Current affiliation: Ehime Prefectural Central Hospital, Matsuyama, Ehime, Japan.

3Current affiliation: Kochi Medical School, Nankoku, Kochi, Japan.

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